益气养阴活血中药对糖尿病视网膜病变大鼠血ET-1及VEGF含量的影响

Effect of Replenishing Qi and Nourishing Yin for Activating Blood Traditional Chinese Medicine on Blood ET-1 and VEGF Content in Diabetic Retinopathy Rats

目的观察益气养阴活血中药对糖尿病大鼠血清胰岛素、ET-1、VEGF含量及视网膜组织病理学的影响,明确益气养阴活血中药对糖尿病视网膜病变的治疗作用机制。方法随机分为空白对照组、模型4周组、模型12周组、给药4周组、给药12周组,每组10只大鼠。模型对照组、空白组灌胃给予蒸馏水,益气养阴活血方组每天上午灌服用蒸馏水稀释的益气养阴活血方,为18 g生药/kg,10 ml,分别于给药后4周,模型对照4周组及益气养阴活血方4周组进行标本采集,对模型12周组和给药12周组给药后12周用同样的方法进行血液及眼球标本采集,采用ELISA法检测血清胰岛素、ET-1、VEGF含量,进行HE染色,数字共焦显微镜下观察大鼠视网膜组织结构。结果与空白对照组比较,造模后4周、12周各组大鼠血清胰岛素水平差异统计学无意义(P>0.05),与空白对照组比较,造模后4周、12周大鼠血清ET-1、VEGF水平均明显升高,(P<0.01或P<0.05);益气养阴活血方给药12周能明显降低糖尿病模型大鼠血清ET-1、VEGF水平(P<0.01),差异具有统计学意义。结论益气养阴活血中药可通过下调血清VEGF及ET-1水平,预防糖尿病视网膜病变的发生和发展,无升高血清胰岛素作用,糖尿病视网膜病变运用益气养阴活血中药治疗糖尿病视网膜病变,需要长期服用,短期服用治疗效果不明显。

Objective To observe the effects of replenishing qi and nourishing yin for activating blood traditional Chinese medicine on serum insulin,ET-1,VEGF content and retinal histopathology in diabetic rats,and to objective clarify the therapeutic mechanism.Methods The rats were randomly divided into blank control group,model 4 weeks group,model 12 weeks group,administration 4 weeks group,12 weeks group,10 rats in each group.The model control group and blank group were given distilled water by gavage,while the replenishing qi and nourishing yin for activating blood traditional Chinese medicine was given replenishing qi and nourishing yin for activating blood traditional Chinese medicine diluted by distilled water every morning,with a volume of 18g crude drug/kg,The blood and eyeball samples were collected at the end of the 12th week in the model control group and the replenishing qi and nourishing yin for activating blood traditional Chinese medicine decoction group at the end of the 4th week after the intervention.The serum insulin,ET-1 and VEGF levels were detected by ELISA,and the HE staining was performed under the digital confocal microscope.The structure of retina was observed.Results Compared with the blank control group,there was no significant difference in serum insulin level between the four and twelve weeks after modeling(P>0.05).Compared with the blank control group,the levels of serum ET-1 and VEGF were significantly increased(P<0.01 or P<0.05).replenishing qi and nourishing yin for activating blood traditional Chinese medicine recipe could significantly reduce the levels of serum ET-1 and VEGF in diabetic rats after 12 weeks The difference was statistically significant(P<0.01).Conclusion The replenishing qi and nourishing yin for activating blood traditional Chinese medicine can prevent the occurrence and development of diabetic retinopathy by down regulating the level of VEGF and ET-1,without increasing the effect of serum insulin.