低谷蛋白大米在仿生大肠反应器中对肠道菌群结构及代谢的影响

Effects of flow glutelin rice on composition and metabolism of intestinal flora in bionic large intestinal reactor

以淀粉、低谷蛋白大米和普通大米为碳源,通过在仿生大肠反应器中对健康人群的粪便微生物进行发酵,探究低谷蛋白大米对人体肠道菌群结构和代谢的影响。结果表明,低谷蛋白大米的有益代谢产物中,短链脂肪酸(short chain fatty acids,SCFAs)的浓度达到104.85 mmol/L,比淀粉组和普通大米组提高44.94%和25.33%,乳酸的浓度达到18.19 mmol/L,比淀粉组和普通大米组提高60.55%和25.28%;低谷蛋白大米的有害代谢产物中,支链脂肪酸(branched chain fatty acids,BCFAs)的浓度为0.29 mmol/L,比普通大米组降低79.31%,氨的浓度为2.60 mmol/L,比普通大米组降低16.15%。平板计数显示,肠道有益菌拟杆菌和双歧杆菌均有显著增长(P<0.05)。16S rDNA测序显示,肠道有益菌拟杆菌门和厚壁菌门的丰度提高,肠道有害菌变形菌门和梭杆菌门的丰度降低。研究结果表明,低谷蛋白大米对健康人群肠道菌群的结构和代谢均具有正向影响。

As a new kind of functional food,the effects of low glutelin rice on intestinal flora after gastrointestinal digestion remained to be studied.Starch,low glutelin rice and ordinary rice were used as carbon sources.The fecal microorganisms of healthy people were fermented in a bionic large intestinal reactor,to explore the effects of low glutelin rice on the structure and metabolism of human intestinal flora.The results showed that in the beneficial metabolites of low glutelin rice,the concentration of short chain fatty acids(SCFAs)reached 104.85 mmol/L,which was 44.94%and 25.33%higher than starch and ordinary rice.The concentration of lactic acid reached 18.19 mmol/L,which was 60.55%and 25.28%higher than starch and ordinary rice.In the harmful metabolites of low glutelin rice,the concentration of branched chain fatty acids(BCFAs)was 0.29 mmol/L,which was 79.31%lower than ordinary rice.The concentration of ammonia was 2.60 mmol/L,which was 16.15%lower than ordinary rice.Plate counting showed that the beneficial intestinal bacteria Bacteroides and Bifidobacterium increased significantly(P<0.05).16S rDNA sequencing showed that the abundance of Bacteroidetes and Firmicutes increased while that of Proteobacteria and Fusobacteria decreased.Based on the results,low glutelin rice has a positive effect on the structure and metabolism of intestinal flora in healthy people.