体外模拟消化对牦牛骨胶原蛋白肽抗氧化活性的影响

Effects of in vitro simulated digestion on the antioxidant activity of yak bone collagen peptides

牦牛骨胶原蛋白经酶解产生的牦牛骨胶原蛋白肽有良好的抗氧化性,为评估其是否适合应用于营养品或保健品的开发,需要研究胃肠道消化对牦牛骨胶原蛋白肽抗氧化活性的影响。研究使用胃蛋白酶、胰液等物质对牦牛骨胶原蛋白肽进行体外模拟胃肠消化,采用水解度、分子质量分布、氨基酸组成对模拟消化前后牦牛骨胶原蛋白肽进行表征,并测定样品·OH清除能力、Fe^(2+)螯合能力及ABTS阳离子自由基清除能力以评估模拟消化对样品抗氧化能力的影响。结果显示,经过体外模拟胃肠消化后,对抗氧化性能起决定性作用的疏水氨基酸含量并无显著变化。分子质量>1000 Da的组分显著降低,且降幅超过50%;<500 Da的组分显著升高,分子质量介于180~1000 Da的主体部分无明显变化。消化前后除·OH清除能力无明显差异外,其他抗氧化性均有提高,表明严苛的胃肠消化不能破坏牦牛骨的抗氧化性功能肽。因此牦牛骨胶原蛋白肽适合应用于营养品和保健品。

Yak bone collagen peptide(YBCP)produced by enzymatic hydrolysis of yak collagen protein has good antioxidant activity.To evaluate whether it is suitable for the development of nutrition or health care products,it is necessary to study the effect of gastrointestinal digestion on the antioxidant activity of YBCP.In this study,the degree of hydrolysis,molecular weight distribution,the amino acid composition of YBCP was characterized before and after simulated digestion.Moreover,the ability of hydroxyl radicals(·OH)scavenging,Fe 2+chelating and ABTS+scavenging was measured to evaluate the effects of simulation digestion on the antioxidant capacity of YBCP.The results showed that the contents of hydrophobic amino acids,which play a decisive role in antioxidation,however,did not change significantly after simulated gastrointestinal digestion.The molecular weight>1000 Da components decreased significantly that the average reduction ratio was more than 50%.The composition<500 Da was significantly increased,and the main part of the molecular weight between 180-1000 Da had no significant changes.There was no significant difference in·OH scavenging ability before and after digestion,but other antioxidant activities were increased.This indicated that severe gastrointestinal digestion could not destroy the antioxidant functional YBCP.Therefore,YBCP is suitable for the application of nutrition and health products.