摘要
The transition to reproductive development is a key step in the lifecycle of a plant and requires fine-tuning of gene expression through the concerted action of several transcription factors(TFs).While most TFs bind naked DNA(without nucleosomes)in a sequence-specific manner,in mammals a special group with key roles in cell fate reprogramming,named pioneer TFs,can bind nucleosome-occupied DNA.In Arabidopsis,LEAFY(LFY)promotes floral fate through activation of floral homeotic genes(Parcy et al.,1998),some of which are targeted by the Polycomb Repressive Complex 2 and localize to closed chromatin environments.Hence,LFY has been proposed to act as a pioneer TF,but whether/how it can bind occluded DNA remained unknown.
