胃癌组织中α肌动蛋白4表达及其对胃癌AGS细胞迁移的影响

Expression of actinin alpha 4 in gastric cancer and its effect on migration of AGS cells

目的探讨胃癌(GC)组织中α肌动蛋白4(ACTN4)的表达及其对GC细胞系AGS细胞迁移的影响。方法采用癌症基因组图谱(TCGA)数据库预测ACTN4在GC中的表达及ACTN4与GC淋巴结转移、肿瘤分期之间的相关性,采用Kaplan-Meier Plotter数据库对ACTN4表达水平在GC中的预后价值进行评估;取对数生长期的GC细胞系AGS、MKN45、HGC-27、MGC803、胃正常黏膜细胞系GES-1与20例GC患者的GC组织及其癌旁组织,采用荧光定量聚合酶链式反应(PCR)检测细胞系和组织中ACTN4 mRNA及蛋白表达;慢病毒构建GC ACTN4敲低模型,采用划痕实验检测AGS细胞迁移能力。结果数据库数据分析发现ACTN4在GC组织中高表达且表达水平与GC淋巴结转移及肿瘤分期呈正相关(P<0.05);Kaplan-Meier Plotter数据库分析表明ACTN4与GC预后不良呈正相关(P<0.05);ACTN4在AGS细胞系中高于GES-1、在GC组织中高于癌旁组织(P<0.05),敲低ACTN4后GC细胞系AGS的迁移能力减弱(P<0.05)。结论ACTN4在GC中高表达且促进AGS细胞的迁移。

Objective To investigate the expression of actinin alpha 4(ACTN4)in gastric cancer(GC)cell lines by bioinformatics and to analyze the effect of ACTN4 in the migration of GC cell lines AGS.Methods The expression of ACTN4 in GC and its correlation with lymph node metastasis and tumor stage in GC were analyzed by bioinformatics analysis from TCGA database.The relationship between expression of ACTN4 and prognosis of GC was predicted by Kaplan-Meier plotter database;The expression of ACTN4 mRNA and protein were detected by fluorescence quantitative PCR in gastric cancer and adjacent tissues and GC cell lines AGS,MKN45,HGC-27,MGC803,GES-1;The ACTN4 knockdown model was constructed by lentivirus,and the migration ability of AGS cells was detected by wound healing assay after knockdown ACTN4.Results By using the data analysis from public database,we found that ACTN4 was highly expressed in gastric cancer tissues.The data analysis results showed that the high expression of ACTN4 was positively correlated with lymph node metastasis and tumor analysis of gastric cancer(P<0.05),and the Kaplan-Meier plotter database analysis showed that ACTN4 was positively correlated with poor prognosis of gastric cancer(P<0.05).The results showed that actn4 was higher in AGS cell line than GES-1,and higher in GC tissue than adjacent tissues(P<0.05).The migration ability of gastric cancer cell line AGS decreased after knockdown ACTN4(P<0.05).Conclusion ACTN4 is highly expressed in gastric cancer and promotes the migration of AGS.