野生黑果枸杞组培快繁体系的建立

Establishment of Rapid Propagation System of Lycium ruthenicum Murray Tissue Culture

黑果枸杞属于茄科枸杞属多年生灌木植物,是重要的荒漠绿化环境改良植物。将黑果枸杞约10 d的无菌苗接种于MS-1愈伤诱导培养基(MS+0.5~1 mg/L NAA+1.5~2.5 mg/L 6-BA+30 g/L蔗糖+8 g/L琼脂,pH=5.2)中,待40 d后,将愈伤组织从外植体上剥离下来后置于MS-2培养基(MS+0.5~1 mg/L NAA+0.5~1.4 mg/L 6-BA+30 g/L蔗糖+6 g/L琼脂, pH=5.2)中以诱导分化不定芽,最后将小芽接种于MS培养基中生根,大约20 d后,幼苗长大,每棵幼苗均有3~4条根,然后进行炼苗和移栽。此愈伤诱导方法由于直接从黑果枸杞的无菌苗获得愈伤组织,极大地缩短了组织培养的时间,减少了培育成本,同时有效提高了培育效率。

Lycium ruthenicum is a perennial shrub of Lycium in Solanaceae,which is an important plant for improving the environment of desert greening.The sterile seedlings of Lycium barbarum were inoculated in callus induction medium MS-1(MS+0.5~1 mg/L NAA+1.5~2.5 mg/L 6-BA+30 g/L sucrose+8 g/L agar,pH=5.2)for about 10 days.After 40 days,the callus was separated from the explants and placed in MS-2 medium(MS+0.5~1 mg/L NAA+0.5~1.4 mg/L 6-BA+30 g/L sucrose+6 g/L agar,pH=5.2)to induce adventitious buds.Finally,the buds were inoculated in MS medium to take root.Rooting in MS medium about 20 days later,the seedlings grew up,each seedling had 3~4 roots,and then carried out seedling refining and transplanting.This method of callus induction is due to obtaining callus directly from sterile seedlings of Lycium barbarum.And it shortens the time of tissue culture,reduces the cost of culture and improves the efficiency of culture in Lycium barbarum.