摘要
Sesquiterpenoids are very common among natural products.A large number of sesquiterpene synthase genes have been cloned and functionally characterized.However,until now there is no report about the d-cadinol synthase predominantly forming d-cadinol(syn.torreyol)from farnesyl diphosphate.Sesquiterpenoids boreovibrins structurally similar to δ-cadinol were previously isolated from culture broths of the basidiomycete fungus Boreostereum vibrans.This led us to expect a corresponding gene coding for a δ-cadinol synthase that may be involved in the biosynthesis of boreovibrins in B.vibrans.Here we report the cloning and heterologous expression of a new sesquiterpene synthase gene from B.vibrans.The crude and purified recombinant enzymes,when incubating with farnesyl diphosphate as substrate,gave δ-cadinol as its principal product and thereby identified as a δ-cadinol synthase.Graphical Abstract A new sesquiterpene synthase gene was cloned from the basidiomycete fungus Boreostereum vibrans and heterologously expressed in E.coli.The purified recombinant enzyme gave δ-cadinol as its principal product from farnesyl diphosphate and thereby identified as a δ-cadinol synthase(BvCS).
基金
This work was supported by a grant from the National Natural Science Foundation of China(21572237).
