摘要
将体质量(6.77±0.51)g凡纳滨对虾饲养在200 L玻璃纤维桶中,每桶50尾,设置2个温度(恒温30℃和1 h内由30℃急升至33℃),每个温度设置以氯化铵溶液调节的4个氨氮质量浓度(0、5、15、25 mg/L),共8个试验组,每组3个平行。胁迫72 h时测定鳃中Na^(+)/K^(+)-ATP酶与Ca^(2+)-ATP酶活性以及肌肉和鳃中游离氨基酸含量,分析高温与氨氮复合胁迫对凡纳滨对虾渗透压生理调节的影响。试验结果显示:(1)同一温度下,30℃时,25 mg/L氨氮组的Na+/K+-ATP酶活性和5 mg/L氨氮组的Ca^(2+)-ATP酶活性均显著升高,而33℃时,3个氨氮胁迫组Na^(+)/K^(+)-ATP酶和Ca^(2+)-ATP酶活性均显著升高(P<0.05);(2)同一氨氮质量浓度下,30℃和33℃试验组的Na^(+)/K^(+)-ATP酶和Ca^(2+)-ATP酶活性均差异显著,且25 mg/L氨氮质量浓度下33℃试验组酶活性显著低于30℃试验组(P<0.05);(3)氨氮质量浓度为15 mg/L和25 mg/L时,33℃试验组鳃和肌肉游离氨基酸总含量均显著高于30℃组(P<0.05);(4)30℃和33℃试验组与氨氮胁迫下,肌肉中丙氨酸含量均随氨氮质量浓度升高而显著增加,且33℃试验组显著高于30℃组(P<0.05)。试验结果表明,高温与氨氮复合胁迫对凡纳滨对虾Na^(+)/K^(+)-ATP酶和Ca^(2+)-ATP酶活性以及游离氨基酸含量影响显著,会造成凡纳滨对虾渗透压生理调节功能紊乱。
The changes in Na^(+)/K^(+)-ATPase and Ca^(2+)-ATPase activities in gill and the contents of free amino acid(FAA)in muscle and gill were monitored in Pacific white shrimp Litopenaeus vannamei with body weight of(6.77±0.51)g reared in 200 L fiberglass barrels at initial stocking density of 50 per barrel under two temperatures(normally 30℃and acute increase from 30℃to 33℃within 1 h)×4 ammonia nitrogen concentrations(0,5,15,and 25 mg/L)adjusted with ammonium chloride solution for 72 h to evaluate the effects of ammonia-nitrogen stress on the physiological osmoregulation of Pacific white shrimp under acute warming temperature.The results showed that the activities of Na^(+)/K^(+)-ATPase and Ca^(2+)-ATPase were found to be significantly increased in 25 mg/L and 5 mg/L ammonia groups at 30℃(P<0.05),significant increase in activities of Na^(+)/K^(+)-ATPaes and Ca^(2+)-ATPase in all ammonia groups at 33℃(P<0.05).In the same ammonia concentration,there were significant differences in the activities of Na^(+)/K^(+)-ATPase and Ca2+-ATPase between 30℃and 33℃groups,significantly lower enzyme activity in the 33℃group than that in the 30℃group at 25 mg/L ammonia concentration(P<0.05).In gill and muscle,the total contents of FAA were significantly lower in the 33℃group than that in 30℃group at ammonia concentration of 15 mg/L and 25 mg/L(P<0.05).Under ammonia stress,alanine level in muscle was increased significantly in 30℃and 33℃groups with increasing ammonia concentration(P<0.05),and significantly higher at 33℃than at 30℃group(P<0.05).The findings indicated that ammonia stress sharply affected Na^(+)/K^(+)-ATPase and Ca^(2+)-ATPase activities as well as the contents of FAA under acute warming temperature,leading to dysfunction of the physiological osmoregulation of Pacific white shrimp.
作者
熊大林
段亚飞
陈成勋
孙永旭
董宏标
詹爱军
张家松
XIONG Dalin;DUAN Yafei;CHEN Chengxun;SUN Yongxu;DONG Hongbiao;ZHAN Aijun;ZHANG Jiasong(Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China;Shenzhen Academy of Inspection and Quarantine, Shenzhen 518010, China)
出处
《水产科学》
CAS
CSCD
北大核心
2021年第4期475-482,共8页
Fisheries Science
基金
国家自然科学基金资助项目(31902343)
中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助项目(2019TS18)
中国水产科学研究院基本科研业务费资助项目(2021XT0604)
广东省渔港建设和渔业产业发展专项项目(A201701B09)
广州市农村科技特派员项目(GZKTP201813).