Jatrophane Diterpenoids from the Seeds of Euphorbia peplus with Potential Bioactivities in Lysosomal-Autophagy Pathway

Euphopepluanones F−K(1−4),four new jatrophane type diterpenoids were isolated from the seeds of Euphorbia peplus,along with eight known diterpenoids(5−12).Their structures were established on the basis of extensive spectroscopic analysis and X-ray crystallographic experiments.The new compounds 1−4 were assessed for their activities to induce lysosomal biogenesis through LysoTracker Red staining.Compound 2 significantly induced lysosomal biogenesis.In addi-tion,compound 2 could increase the number of LC3 dots,indicating that it could activate the lysosomal-autophagy pathway.