乳腺浸润性导管癌中SATB1、NF-κB p65及HER-2蛋白的表达及其与临床病理的相关性

SATB1,NF-κB and HER-2 Expression Patterns in Invasive Ductal Breast Carcinoma and Their Correlation with Clinical and Pathological Parameters

【目的】探讨乳腺浸润性导管癌中特异性核基质结合区结合蛋白1(special AT-rich binding protein 1,SATB1)、核转录因子-κB p65(nuclear transcription factor-kappa B,NF-κB p65)及人上皮生长因子受体2(human epidermal growth factor receptor 2,HER-2)的表达及其与临床病理参数的相关性。【方法】利用免疫组化检测90例乳腺浸润性导管癌及癌旁组织中SATB1、NF-κB及HER-2蛋白的表达;对HER-2蛋白中度阳性者利用荧光原位杂交(fluorescence in situ hybridization,FISH)检测HER-2基因的扩增。分析SATB1、NF-κB p65及HER-2的表达与临床病理参数的相关性。【结果】SATB1、NF-κB p65及HER-2蛋白在乳腺癌组织中的阳性表达率分别为71%(64/90)、53.3%(48/90)和30%(27/90),明显高于癌旁组织。SATB1和NF-κB p65蛋白表达与肿瘤大小、组织学分级、TNM分期、腋窝淋巴结转移及雌激素受体(estrogen receptor,ER)表达状态有关,而与患者年龄无关。Her-2基因过表达仅与腋窝淋巴结转移有关(P<0.05),而与肿瘤大小、组织学分级、TNM分期、ER表达状态及年龄无关。在乳腺浸润性导管癌中SATB1和NF-κB p65蛋白的表达呈显著正相关,HER-2的表达与SATB1和NF-κB p65蛋白之间均未见明显相关性。【结论】SATB1和NF-κB p65蛋白表达与浸润性导管癌的临床病理参数密切相关,它们在乳腺癌的发生发展中具有协同作用,有可能作为乳腺浸润性导管癌的预后指标。

【Objective】To investigate the expression patterns of Special AT-rich binding protein 1(SATB1),nuclear factor-kappa B p65(NF-κB p65)and HER-2 in invasive ductal breast carcinoma,and their correlation with the clinical and pathological Parameters.【Methods】The protein expression of SATB1,NF-κB p65 and HER-2 were examined by immunohistochemistry,and fluorescence in situ hybridization(FISH)was employed to detect HER2 amplification.【Results】The expression rates of SATB1,NF-κB p65 and HER-2 in 90 cases of invasive ductal breast carcinoma were 71%(64/90),53.3%(48/90)and 30%(27/90)respectively,and higher in comparison to normal breast tissues.The expression of SATB1 and NF-κB p65 were correlated with tumor size,histological classification,TNM stage,lymph node metastasis and ER status,and not associated with age.The over-expression of HER-2 was only correlated with lymph node metastasis,but not associated with tumor size,histological classification,TNM stage,ER status and age.The positive correlation was found between the expression of SATB1 and NF-κB,but HER2 expression was not significantly correlated with that of other proteins.【Conclusion】The expression of SATB1 and NF-κB were closely correlated with clinical and pathological parameters of invasive ductal breast carcinoma,and may have synergistic effect in during the occurrence and development of breast carcinoma.The expression of SATB1 and NF-κB may be used as prognostic biomarkers of invasive ductal breast carcinoma.