急性胰腺炎患者血清内源性生长激素释放肽与氧化应激介质的相关性研究

Study on the Correlation of Serum Endogenous Growth Hormone Releasing Peptide and Oxidative Stress Media in Patients with Acute Pancreatitis

【目的】检测急性胰腺炎(AP)患者血清内源性生长激素释放肽(Ghrelin)与对氧磷酶-1(PON-1)、超氧化物歧化酶(SOD)和丙二醛(MDA)等氧化应激介质水平的变化及其相关性。【方法】选取195例AP患者作为研究对象(观察组),其中轻度106例(轻度组)、中度59例(中度组)、重度30例(重度组)。另选取健康体检者45例作为对照(对照组)。健康体检者在体检当天,AP患者在入院d1、d3和d7空腹采集静脉血进行内源性Ghrelin、PON-1、SOD和MDA检测,分析各组对象上述指标水平的变化及内源性Ghrelin与PON-1、SOD、MDA相关性。【结果】AP患者血清内源性Ghrelin、PON-1和SOD水平随着病情程度的缓解其水平逐渐下降且均低于对照组,而血清MDA水平则正好相反,各组间比较有统计学意义(P<0.05)。随着入院时间的延长,AP患者血清内源性Ghrelin、PON-1和SOD水平随着病情的好转而逐渐升高,而血清MDA水平则逐渐降低,同组间不同时间点比较具有统计学意义(P<0.05)。Spearman相关性显示:AP患者血清内源性Ghrelin与PON-1、SOD呈正相关性(r值分别为0.335和0.414,P<0.001),而与MDA呈负相关性(r=-0.393,P<0.001)。【结论】血清内源性Ghrelin水平随着AP患者病情的加重而呈降低趋势,并与PON-1、SOD、MDA等氧化应激介质相关,为AP的早期诊断、病情严重程度判断和寻找新的治疗靶点提供了依据。

【Objective】To detect the changes of serum endogenous growth hormone releasing peptide(Ghrelin),paraoxonase-1(PON-1),superoxide dismutase(SOD)and malondialdehyde(MDA)in patients with acute pancreatitis(AP).【Methods】A total of 195 patients with AP were selected as the research object(observation group),including 106 mild cases(mild group),59 moderate cases(moderate group)and 30 severe cases(severe group).Another 45 healthy people were selected as control group.On the day of physical examination,venous blood samples were collected from patients with AP on D1,D3 and D7 for detection of endogenous Ghrelin,PON-1,SOD and MDA.The changes of the above indexes and the correlation between endogenous Ghrelin and PON-1,SOD and MDA were analyzed.【Results】The levels of serum endogenous Ghrelin,PON-1 and SOD in AP patients gradually decreased with the remission of the disease,and were lower than those in the control group,while the level of serum MDA was just the opposite,and the comparison among the groups was statistically significant(P<0.05).With the extension of admission time,the serum endogenous Ghrelin,PON-1 and SOD levels of AP patients gradually increased with the improvement of the disease,while the serum MDA level gradually decreased,with statistical significance at different time points in the same group(P<0.05).Spearman correlation showed that serum endogenous Ghrelin was positively correlated with PON-1 and SOD(r=0.335 and 0.414,P<0.001),and negatively correlated with MDA(r=-0.393,P<0.001).【Conclusion】The level of serum endogenous Ghrelin decreases with the aggravation of AP,and is related to the oxidative stress mediators such as PON-1,SOD and MDA,which provides a basis for the early diagnosis of AP,the judgment of disease severity and the search for new therapeutic targets.