荧光共轭聚合物纳米微球的制备及光动力抗菌研究

Study on preparation and photodynamic antibacterial property of fluorescent conjugated polymer nanosphere

通过水包油的微乳液聚合法,以异丙基丙烯酰胺和甲基丙烯酰氧乙基三甲基氯化铵为共聚单体,并与荧光共轭聚合物(FCP)混合后合成荧光共轭聚合物纳米微球(FCP-NPs),使用红外光谱、扫描电镜、透射电镜激光粒度仪等手段对微球进行了表征。结果表明:聚合物纳米微球包载了FCP,其平均粒径大约为119±13nm。FCP-NPs溶液在532nm激光照射下能生成单线态氧,具有光动力杀菌潜力。用FCP-NPs溶液分别与大肠杆菌、金黄色葡萄球菌及多重耐药菌耐甲氧西林金黄色葡萄球菌共培养,FCP-NPs在浓度较高时(>5mg/mL),激光照射下对于两种常见菌株的抑菌率大于95%,对耐药菌的抑菌率约80%。在无光照或者无FCP情况下,纳米微球仍然有一定的抑菌性能,因此FCP-NPs具有光动力-铵阳离子协同杀菌的功能。

The oil-in-water microemulsion polymerization was used to synthesize fluorescent polymer nanomicrospheres(FCP-NPs)with N-isopropylacrylamide and 2-(methacryloyloxy)ethyl trimethylammonium chloride as monomers mixed with a self-synthesized conjugated polymer.FCP-NPs were characterized by FT-IR,SEM,TEM,and laser particle size analyzer.The results showed that conjugated polymer was immobilized successfully in the nanospheres with the average size of 119±13 nm in diameter.The FCP-NP solution was found generating singlet oxygen after exposing to 532 nm laser light showing photodynamic antibacterial ability.Thus E.coli,S.aureus and multi-antibiotic resistant bacteria MRSA were used for test.The results indicated that the inhibition rate of FCP-NPs to two common bacterial strains was more than 95%,and to MRSA was about 80%when the concentration of FCP-NPs was high(>5 mg/mL).In the absence of light or FCP,the nano microspheres still showed antibacterial properties.Therefore,FCP-NPs had the function of photodynamic-ammonium cation synergistic sterilization.