摘要
Mammalian germ line cells undergo unique cellular and genetic changes under the regulation of specific regulators,in different stages as they develop and differentiate into functional gametes.It is a fundamental challenge to reconstitute gametes development in vitro,because of the complexity of the regulation process.In mice,embryonic stem cells(ESCs)develop into Epiblast stem cells at around embryonic day 6.0(E6.0)induced by the bone morphogenetic protein 4(Bmp4)(Lawson et al.,1999).At around E7.25,epiblast stem cells develop into primordial germ cells(PGCs)in the extraembryonic mesoderm regulated by the critical transcriptional regulator Blimp1(Prdm1)and Prdm14(Yamaji et al.,2008).PGCs are the origins for the oocytes and the spermatozoa(a motile sperm cell).
