赵进喜教授治疗2型糖尿病的核心处方挖掘及生物学机制探讨

Data mining and biological mechanism exploration of Professor ZHAO Jinxi’s core prescription in the treatment of type 2 diabetes mellitus

[目的]应用数据挖掘及网络药理学技术,对赵进喜教授治疗2型糖尿病(T2DM)药物的核心配伍、潜在靶点、作用机制进行探讨和研究。[方法]收集325例赵进喜教授诊治2型糖尿病的医案,应用关联规则算法筛选核心药物配伍。使用中药系统药理学数据库与分析平台(TCMSP)筛选核心处方的活性成分,应用STITCH、TTD、DrugBank、GAD和DisGeNET数据库,筛选药物及T2DM相关靶点。采用Cytoscape中的MCODE算法识别功能模块,应用ClueGO插件进行功能分析。[结果]通过关联分析得到黄芩、葛根、丹参、仙鹤草、荔枝核、地骨皮等6味药物的核心处方。通过网络药理学分析,得到58个药物-疾病相关靶点,识别出5个功能模块。对重要靶点进行KEGG通路分析,得到18条相关通路。[结论]核心处方治疗T2DM是多成分、多靶点、多环节、协同作用的过程,其作用靶点可能为SLC2A2、SLC2A4、TNF,PPARA和PPARG,并可以通过调节脂肪因子信号通路和PPAR信号通路发挥作用。

[Objective]Based on the data mining and network pharmacology technology,the core compatibility,potential targets and action mechanism of Professor ZHAO Jinxi’s drugs for treating type 2 diabetes mellitus(T2DM)were discussed and studied.[Methods]The medical records of 325 cases of T2DM treated by Professor ZHAO Jinxi were collected,and the compatibility of core drugs was screened by association rules algorithm.TCMSP platform was used to screen the active ingredients of core prescriptions,and STITCH,TTD,DrugBank,GAD and DisGeNET databases were used to screen drugs and T2DM-related targets.MCODE algorithm in Cytoscape is used to identify the functional modules,and ClueGO plug-in is used to analyze the functions.[Results]The core combination of 6 drugs including Scutellaria baicalensis Georgi,Radix Puerariae,Salvia miltiorrhiza Bunge,Agrimonia pilosa,Lychee Seed and Cortex Lycii was screened out by correlation analysis.Through network pharmacological analysis,58 drug-disease related targets were obtained and 5 functional modules were identified.The 18 related pathways were obtained by KEGG pathway analysis of important targets.[Conclusion]The treatment of T2DM with core prescription is a multi-component,multi-target,multi-link and synergistic process.Its target may be SLC2A2,SLC2A4,TNF,PPARA and PPARG,and it can play a role by regulating fat factor signal pathway and PPAR signal pathway.