基于尿液代谢组学探讨咸味致病的生物学机制

Study on The Biological Mechanism of Diseases due to Salty Taste Based on Urine Metabonomics

目的:研究高盐饮食对大鼠尿液代谢组学的影响,探讨咸味致病的潜在生物学机制。方法:将16只SD大鼠随机分为高盐饮食组和正常对照组各8只,高盐饮食组给予高盐饲料(8%Nacl浓度)饲养,正常对照组给予普通饲料(0.4%Nacl浓度)饲养,60 d后收集2组的尿液样本,运用基于气相色谱-质谱联用(gas chromatography mass spectrometer,GC-MS)的代谢组学技术进行研究。结果:PCA与OPLS-DA得分图显示,2组的代谢轮廓存在显著差异。与正常对照组比较,高盐饮食组中有28种代谢产物表达水平出现改变,差异代谢产物主要涉及半乳糖代谢、丙氨酸与天冬氨酸和谷氨酸代谢、嘧啶代谢、淀粉和蔗糖代谢、泛酸和辅酶A生物合成、三羧酸循环等6条代谢路径。结论:过食咸味影响机制涉及扰动机体的糖代谢、能量代谢、嘧啶代谢、脂质代谢等多个层面,是一个多因素、多层次的复杂过程。

Objective:It is to study the effect of high salt diet on rat urine metabolomics and explore the potential biological mechanism of diseases due to salty taste.Methods:16 SD rats were randomly divided into the high salt diet group(8)and normal control group(8).High salt diet group was fed with high salt concentration(8%Nacl),while normal control group was fed with common salt concentration(0.4%Nacl).After breeding the two groups for 60 days,urine samples of the two groups were collected,and metabonomics technique was studied based on Gas Chromatography Mass Spectrometry(GC-MS).Results:PCA and OPLS-DA score plots showed significant differences in metabolic profiles between the two groups.Compared with the normal control group,the expression levels of 28 metabolites in high-salt diet group were changed.The differential metabolites were mainly involved with 6 metabolic pathways including galactose metabolism;alanine,aspartate and glutamate metabolism;pyrimidine metabolism;starch and sucrose metabolism;pantothenate and coa biosynthesis;and citrate cycle.Conclusion The influence mechanism of overeating salty taste is involved with multiple levels like the disturbance of glucose metabolism,energy metabolism,pyrimidine metabolism,lipid metabolism and so on.It is a multi-factor and multi-layered complex process.