穴位埋线对变应性鼻炎大鼠炎症介质、细胞因子及JNK通路的调节作用

Regulation Effect of Acupoint Catgut Embedding on Inflammatory Mediators,Cytokines and JNK Pathway in Rats with allergic rhinitis

目的:探讨穴位埋线对变应性鼻炎(AR)大鼠炎症介质、细胞因子及c-jun氨基端激酶(JNK)通路的调节作用。方法:SD大鼠随机分为对照组、模型组、西药组、埋线组,除对照组外均采用卵清蛋白+氢氧化铝致敏及卵清蛋白激发的方式建立AR模型,造模后西药组给予丙酸倍氯米松滴鼻治疗,埋线组给予"百会""肺俞""脾俞"穴埋线治疗连续4周,治疗后比较4组间鼻黏膜病理改变、血清和鼻黏膜中免疫球蛋白E(IgE)、嗜酸性粒细胞趋化蛋白(Eotaxin)、干扰素-γ(IFN-γ)及白介素(IL)-4、-5、-17、磷酸化氨基末端蛋白激酶(p-JNK)的差异。结果:与对照组比较,模型组鼻黏膜出现了充血水肿及炎症细胞浸润等明显的病理改变,IgE、Eotaxin、IL-4、IL-5、IL-17含量及p-JNK的表达均明显增加,IFN-γ的含量明显降低(P<0.05);与模型组比较,西药组及埋线组鼻黏膜的病理改变明显改善,IgE、Eotaxin、IL-4、IL-5、IL-17的含量及p-JNK的表达均明显降低,IFN-γ的含量明显增加(P<0.05);埋线组与西药组的上述指标差异无统计学意义(P>0.05)。结论:穴位埋线能调节AR大鼠炎症介质及细胞因子的释放,抑制JNK通路激活是介导这一调节作用的可能机制。

Objective:To investigate the regulatory effect of acupoint catgut embedding on inflammatory mediators,cytokines and c-jun-N-terminal kinase(JNK)pathway in rats with allergic rhinitis(AR).Methods:SD rats were randomly divided into control group,model group,western medicine group and embedding group.Besides the control group,AR model was established by ovalbumin+aluminum hydroxide sensitization and ovalbumin stimulation in other 3 groups.After modeling,western medicine group was given beclomethasone propionate nasal drip treatment,embedding group was given acupoint catgut embedding treatment for 4 weeks.After treatment,the differences of the pathological changes of nasal mucosa,inflammatory mediators immunoglobulinE(IgE),eosinophil chemoattractant protein(eotaxin),cytokines interferon-γ(IFN-γ),interleukin(IL)-4,-5,-17,p-JNK were compared among the four groups.Results:compared with the control group,the nasal mucosa of the model group showed obvious pathological changes,including hyperemia,edema and inflammatory cell infiltration,the contents of IgE,eotaxin,IL-4,IL-5,IL-17 and the expression of p-JNK significantly increased,the content of IFN-γsignificantly reduced(P<0.05);compared with the model group,the pathological changes of the nasal mucosa of the western medicine group and the embedding group significantly improved,the contents of IgE,eotaxin,IL-4,IL-5,IL-17 and the expression of p-JNK significantly decreased,the content of IFN-γsignificantly increased(P<0.05)and there was no difference between embedding group and Western medicine group(P>0.05).Conclusion:acupoint catgut embedding can improve the release of inflammatory mediators and cytokines in AR rats,and inhibition of JNK pathway activation is the possible mechanism of this improvement.