好氧反硝化菌的固定化及其效能研究

Immobilization and efficacy of an aerobic denitrifier

为了提高好氧反硝化菌的环境耐受性和脱氮效率,采用聚乙烯醇(PVA)、海藻酸钠(SA)和稻壳粉作为载体对好氧反硝化菌进行固定化,并对固定化颗粒的性能进行评价.结果如下:固定化颗粒最佳配比为12%聚乙烯醇(PVA)、8%海藻酸钠(SA)、0.5 g稻壳粉和10 mL菌液;固定化颗粒具有较好的稳定性和传质性,48 h的总氮(TN)去除率为89.35%~90.12%.固定化颗粒对pH值和转速具有良好的耐受性,pH值为11时,TN去除率为90%;120 r/min时TN和NH_(4)^(+)-N去除率最高,分别为91.29%和93.30%;固定化颗粒不耐低温(10℃和15℃),在10℃时,TN去除率仅为20%左右;但是在30℃时,TN去除率可达90.59%.

To improve the environmental tolerance and nitrogen removal efficiency of an aerobic denitrifier,polyvinyl alcohol(PVA),sodium alginate(SA),and rice hull powder were used as immobilized carriers for an aerobic denitrifier and the performance was subsequently evaluated.The results showed that the optimal ratio of immobilized particles was a mixture of 12%PVA,8%sodium alginate(SA),0.5 g rice hull powder,and 10 mL bacterial solution.The immobilized particles had strong stability and mass transfer capability;the removal efficiency of TN was 89.35%~90.12%over 48h.The immobilized particles had good tolerance to pH and rotating speed.When the pH was 11,the removal efficiency of TN was 90%.The removal efficiency of TN and NH_(4)^(+)-N was the highest(91.29%and 93.30%,respectively)when the speed was 120 r/min.The immobilized particles were not resistant to low temperatures(10℃and 15℃),and the TN removal efficiency was only about 20%at 10℃.The TN removal efficiency,however,achieved 90.59%at 30℃.