中心复合设计-效应面法优化复方延胡索微乳凝胶贴膏中挥发油的提取工艺

Optimization of extraction process for volatile oil from compound Corydalismicroemulsiongel cataplasmaby central composite design-response surface methodology

目的通过中心复合设计-效应面法优化挥发油的提取工艺。方法建立分析方法,采用水蒸气蒸馏法提取挥发油,选取加水量、浸泡时间、提取时间作为考察因素,挥发油收率、蒿本内酯、桂皮醛含量作为优化指标,得出最优处方,并进行验证性实验。结果最佳提取工艺为加10倍水,浸泡100 min,提取8 h,预计挥发油收率为0.413%,蒿本内酯含量39.20%、桂皮醛23.30%,实际值与预测值误差均小于0.05。结论中心复合设计-效应面法用于优选复方延胡索微乳凝胶贴膏挥发油提取工艺准确性高,所得工艺参数合理可靠。

Objective To optimize the extracting condition of volatile oil by central composite design-response surface methodology.Methods Analytical method was established,and volatile oil was extracted by the method of steam distillation.Yield of water,soaking time and extracting time were selected as study factors.The extraction rate of volatile oil,the content of ligustilide and cinnamaldehyde was as the optimized index to abtain the optimal extraction for verifying tests.Results The optimal extracting process was as follows:adding10 times of water,soaking for 100 minutes and extraction time was 8 hours.The expected extraction rate of volatile oil was 0.413%,the content of ligustilide was 39.20%,and cinnamaldehyde was 23.30%.The error between the actual value and the predicted value was less than 0.05.Conclusion The central composite design-response surface methodology has a high accuracy in the extraction of volatile oil,and the parameters are reasonable and reliable.