白细胞介素6/白细胞介素6受体轴免疫调节机制与乙型肝炎病毒感染患者病毒基因型的相关性

The immunoregulatory mechanism of IL-6/IL-6 receptor axis and its correlation with HBV genotype in patients with HBV infection

目的研究白细胞介素6(IL-6)/IL-6受体轴免疫调节机制及其与乙型肝炎病毒(HBV)感染患者HBV基因型的相关性。方法选取我院2017年6月至2019年12月期间收治的96例HBV感染患者为研究组,另选取同期于我院体检健康者为对照组,比较两组血清IL-6、可溶性IL-6R(sIL-6R)水平。感染患者根据基因型主要分为B基因型、C基因型,另外少数A基因、A/C混合型未纳入研究。比较两组患者的IL-6/IL-6受体轴水平以及HBV DNA,采用Pearson相关分析IL-6、sIL-6R与HBV基因型的相关性,采用偏相关分析血清IL-6、sIL-6R水平与HBV DNA水平的相关性。结果研究组血清IL-6、sIL-6R水平明显高于对照组(P <0.05)。C基因型的血清HBV DNA、IL-6/IL-6受体轴水平均明显高于B基因型患者(P <0.05),pearson相关分析显示IL-6、sIL-6R与HBV基因型具有相关性(r=0.543、0.513)。偏相关分析显示Log HBV DNA与IL-6、sIL-6R呈正相关(偏相关系数为0.501、0.459,P <0.05)。结论 IL-6/IL-6受体轴免疫调节与HBV感染密切关联,其中C基因型感染者的IL-6/IL-6受体轴处于高表达状态,且与HBV-DNA载量呈正相关。

Objective To study the immunoregulatory mechanism of interleukin-6(IL-6)/IL-6 receptor axis and its correlation with hepatitis B virus(HBV)genotype in patients with HBV infection.Methods 96 patients with HBV infection treated in our hospital from June 2017 to December 2019 were selected into the study group,and some healthy people in our hospital during the same period were selected into the control group.The serum levels of IL-6 and sIL-6R were compared.According to the genotype,the infected patients were mainly divided into B genotype and C genotype,while a few A genotype and A/C mixed genotype were not included in the study.The levels of IL-6/IL-6 receptor axis and HBV DNA were compared between the two groups.Pearson correlation was used to analyze the correlation between IL-6,sIL-6R and HBV genotypes,and partial correlation was used to analyze the correlation between serum IL-6,sIL-6R and HBV DNA level.Results The serum levels of IL-6 and sIL-6R in the study group were significantly higher than those in the control group(P<0.05).Serum HBV DNA and IL-6/IL-6 receptor axis levels of C genotype were significantly higher than those of B genotype patients(P<0.05).Pearson correlation analysis showed IL-6 and sIL-6R were correlated with HBV genotype(r=0.543,0.513).Partial correlation analysis showed that log HBV DNA was positively correlated with IL-6 and sIL-6R(partial correlation coefficient were 0.501,0.459),(P<0.05).Conclusion The immunomodulation of IL-6/IL-6 receptor axis is closely related to HBV infection.Among them,the IL-6/IL-6 receptor axis of C genotype infection is in a state of high expression,which is positively related to HBV-DNA load.