三七总皂苷调节PDGF-BB/PDGFR-β的表达促进大鼠浅表Ⅱ°烧伤创面愈合

PNS Promotes the Wound-healing of Superficial Second-degree Burn Rats by Regulating PDGF-BB/PDGFR-βExpression

目的观察三七总皂苷(PNS)对大鼠浅表Ⅱ°烧伤创面的治疗作用及对PDGF-BB/PDGFR-β表达的影响,探讨PNS治疗浅表Ⅱ°烧伤的作用机制。方法复制大鼠浅表Ⅱ°烧伤模型,模型复制成功的大鼠用PNS治疗,分别于给药后第0、7、14、21天活体取材,应用免疫组化(IHC),Western blot和RT-PCR检测烧伤皮肤组织中PDGF-BB/PDGFR-β蛋白和mRNA的表达变化。此外,在不同时间点宏观监测皮肤组织恢复情况。结果 HE染色提示,模型复制是成功的。宏观监测数据显示,和模型组相比,PNS高剂量组大鼠脱痂、长毛时间均见缩短,创面愈合率提高(P <0.05)。免疫组化,WB和RT-PCR结果显示,PNS高剂量组的PDGFBB/PDGFR-β的蛋白和mRNA表达在第7天急剧增加,至第14天持续维持在较高水平,第21天接近正常组水平;而模型组表达高峰出现在烧伤后第21天,PDGF-BB/PDGFR-β表达升高是一个缓慢的过程。结论 PNS能明显促进大鼠浅表Ⅱ°烧伤模型皮肤创面的愈合,其机制可能与其上调皮肤组织中的PDGF-BB/PDGFR-β的蛋白和mRNA表达密切相关。

Objective To observe the therapeutic of PNS on wound healing and the expression of PDGF-BB,PDGFR-βin rats superficial second-degree burn model,and to explore the underlying mechanism of PNS on second-degree burn wound healing.Methods Rats with superficial second-degree burn were treated with PNS,applying HE staining to detect burn severity,and then biopsied biological tissue on days 0,7,14,21 after administration,respectively.Moreover,Immunohistochemistry(IHC),Western blot and RT-PCR methods were used to detect protein and mRNA expression levels of PDGF-BB/PDGFR-βin skin tissues after treatment with PNS,respectively.Furthermore,the skin wound healing at different time points was monitored by macroscopic observation.Results HE staining suggested that the replication of rats second-degree burn model was successful.Macro monitoring data demonstrates that compared with model group,the removal time of scab and fur-growthing were shortened,and the wound healing rate was increased in the PNS high-dose group(P<0.05).Combination of IHC,WB and RT-PCR showed that the expression levels of PDGF-BB/PDGFR-βincreased sharply on day 7,maintaining a high level until day 14,approaching normal levels on day 21 after PNS treatment.However,the protein and m RNA expressions of PDGF-BB/PDGFR-βshowed high values on day 21 in the model group,which was a slow process.Our results suggest that compared with model group,the peak expression of PDGF-BB/PDGFR-βare earlier,shortening of fur-growthing and decrustation time,and improvement of wound healing rate in rats superficial second-degree burn model.Conclusion PNS can significantly promote skin wound healing in rats superficial second-degree burn model,and its mechanism is closely related to the up-regulation of the expression levels of PDGF-BB/PDGFR-βin skin tissue.