RANTES及其受体CCR5基因多态性及环境因素在昆明汉族T2DM发生中的交互作用

The Interaction among Gene Polymorphisms of RANTES and Its Receptor CCR5 and Environmental Factors in the Development of T2DM in Kunming Han Nationality

目的探讨RANTES基因启动子区rs2280788位点、CCR5基因启动子区rs1799987位点多态性及环境因素在昆明地区汉族2型糖尿病(Type 2 diabetes mellitus, T2DM)的发生中是否存在交互作用。方法收集92例昆明地区汉族血糖正常者和97例T2DM患者的一般资料及外周静脉血,采用Taqman实时荧光定量PCR检测RANTES基因rs2280788位点及其受体CCR5基因rs1799987位点的多态性,运用多因子降维法(multifactor dimensionality reduction, MDR)分析RANTES及其受体基因多态性与环境因素在昆明地区汉族T2DM的发生中是否存在交互作用。结果 CCR5 rs1799987与RANTES rs2280788间存在交互作用(测试组平衡精度为0.531 4,训练组平衡精度为0.582 0,交叉验证一致性为10/10,P <0.05,OR:2.046 5,95%CI:1.111 8~3.767 2);高血压与中心性肥胖间存在交互作用(测试组平衡精度为0.703 1,训练组平衡精度为0.703 1,交叉验证一致性为10/10,P <0.001,OR:8.164 0,95%CI:3.874 5~17.202 6);未发现CCR5 rs1799987与环境因素、RANTES rs2280788与环境因素间存在交互作用(P> 0.05)。结论 RANTES基因启动子区-28(rs2280788)与CCR5基因启动子区59 029(rs1799987)SNP位点之间、高血压与中心性肥胖之间均存在交互作用,具有交互作用的因素同时存在会增加昆明地区汉族T2DM的患病风险。

Objective To investigate the interaction among RANTES gene promoter region rs2280788 C/G polymorphism,CCR5 gene promoter region rs1799987 G/A polymorphism and environmental factors in the development of Type 2 diabetes mellitus(T2DM)in the Han ethnic group in Kunming.Methods The general data and peripheral venous blood of 92 Han people with normal blood glucose and 97 Han T2DM patients in Kunming area were collected.Polymorphisms of RANTES gene rs2280788 and CCR5 gene rs1799987 were detected by Taq Man real-time quantitative PCR.Multifactor dimensionality reduction(MDR)was used to analyze the interaction among the gene polymorphisms of RANTES and its receptor and environmental factors in the occurrence of T2DM in Han nationality in Kunming.Results There was an interaction between CCR5 rs1799987 and RANTES rs2280788(the testing balance accuracy was 0.5314,the training balance accuracy was 0.5820,and the cross validation consistency was 10/10,P<0.05,OR:2.0465,95%CI:1.1118-3.7672);There was an interaction between hypertension and central obesity(the testing balance accuracy was 0.7031,the training balance accuracy was0.7031,the cross validation consistency was 10/10,P<0.001,OR:8.1640,95%CI:3.8745-17.2026);No interaction between CCR5 rs1799987 and environmental factors,as well as RANTES rs2280788 and environmental factors was found(P>0.05).Conclusions There are interactions between the RANTES gene promoter region-28(rs2280788)and the CCR5 gene promoter region 59029(rs1799987)SNP,as well as between hypertension and central obesity,the co-existence of interacting factors will increase the risk of T2DM in Han nationality in Kunming.