15个产地玄参中哈巴苷与哈巴俄苷含量测定

Determination of harpagide and harpagoside contents in Scrophularia ningpoensis from 15 origins

目的建立HPLC法同时测定玄参中哈巴苷、哈巴俄苷的含量。方法采用HPLC-PDA法测定,固定相采用Eclipse Plus C_(18)色谱柱,以乙腈–0.03%磷酸水溶液为流动相,梯度洗脱;体积流量1.0 ml/min;检测波长210、280 nm。结果哈巴苷、哈巴俄苷分别在0.1020~0.5100 mg/ml(r=0.9999)、0.0340~0.1700 mg/ml(r=0.9999)范围内线性关系良好,平均加样回收率分别为97.44%、97.08%,RSD分别为0.93%、1.24%。不同产地之间哈巴苷与哈巴俄苷含量差异大(P<0.01),其中、四川陇东、浙江临安、磐安、河南南丰含量较高。结论该方法稳定准确,重复性好,可用于玄参的质量控制。

Objective To establish an HPLC method for the simultaneous determination of harpagide and harpagoside content in Scrophularia ningpoensis(SN).Methods An Eclipse C_(18)column was used for determination of methanol extract of S.ningpoensis with a HPLC-PDA method and mobile phase of acetonitrile-0.03%phosphate solution in a gradient elution manner.The flow rate of mobile phase was 1.0 ml/min,and the detection wavelengths were 210 nm and 280 nm.Results Harpagide and harpagoside contents in SN showed good linear relationships within 0.1020-0.5100 mg/ml(r=0.9999)and 0.0340-0.1700 mg/ml(r=0.9999).Their average recovery rates were 97.44%and 97.08%.The RSDs were 0.93%and 1.24%..There were significant differences in the contents of harpagide and harpagoside in SN from 15 origins(P<0.01).The content of harpagoside in Sichuan Long-dong,Zhejiang Lin-an,Zhejiang Pan-an and Henan Nan-feng is higher.Conclusion This method is stable,accurate and reproducible and can be used for the quality control of SN.