总状共头霉CGMCC 3.2500对积雪草酸的生物转化

Microbial transformation of Asiatic acid by Syncephalastrum racemosum CGMCC 3.2500

目的积雪草酸是积雪草中的主要药用成分,为乌苏烷型三萜化合物,具有多种生物活性,通过对其进行结构修饰,以获得活性更好的积雪草酸类似物。方法使用总状共头霉CGMCC 3.2500对积雪草酸进行生物转化,通过高分辨质谱(HR-ESI-MS)、核磁共振波谱(包括^(1)H NMR、^(13)C NMR、^(1)H-^(13)C HSQC、^(1)H-^(13)C HMBC、^(1)H-^(1)H NOESY)鉴定其结构。结果获得一个化合物,最终确定化合物的结构为2-氧-3α,15α,23-三羟基-12-烯-28-油酸,该化合物为新化合物。结论总状共头霉CGMCC 3.2500能够对积雪草酸进行结构修饰,获得积雪草酸类似物。

Objective Asiatic acid is the main medicinal component of aursane pentacyclic triterpene and possessed various biological activities.In order to obtain better active Asiatic acid analogues,microbial transformation was used for structural modification.Methods Asiatic acid was biotransformed by Syncephalum racemosum CGMCC 3.2500.The structure of the compound was identified by high resolution electrospray ionization mass spectroscopy(HR-ESI-MS)and nuclear magnetic resonance spectroscopy(i.e.,^(1)H NMR、^(13)C NMR、^(1)H-^(13)C HSQC、^(1)H-^(13)C HMBC、^(1)H-^(1)H NOESY).Results The structure of the compound was determined as 2-oxo-3α,15α,23-trihydroxyurs-12-en-28-oic acid which was a new compound.Conclusion Syncephalum racemosum CGMCC 3.2500 can modify the structure of Asiatic acid and obtain Asiatic acid analogues.