棉酚锌复合物的合成及其结构解析

Synthesis and structure elucidation of complex of gossypol with zinc

为了探究棉酚锌复合物的结构,在氩气的保护下以甲醇为溶剂,通过棉酚与硫酸锌合成棉酚锌复合物。利用红外光谱和高效液相色谱对复合物进行表征分析。红外光谱的分析结果表明,棉酚分子中的芳醛基完全参与了与Zn2+的螯合。复合物经高效液相色谱分离后得到5个不同于棉酚的峰,表明棉酚锌复合物由5种化合物组成,其中2种化合物通过棉酚与Zn2+以1∶2的摩尔比螯合而成,另外3种化合物分别按棉酚与Zn2+以1∶1、1∶3、1∶4的摩尔比螯合而成。该研究成果能很好地阐释游离棉酚超标的日粮会导致单胃动物缺乏微量元素锌,进而引发一系列缺锌的临床症状。

To explore the structure of complex of gossypol with zinc, the complex was synthesized by reaction of gossypol and zinc sulfate in methanol under the protection of argon atmosphere.The complex was characterized by FT-IR and high performance liquid chromatography(HPLC) separation.The results from infrared spectrum showed that the aromatic aldehyde group in gossypol was completely involved in the chelation with Zn2+.Five peaks different from gossypol were obtained after separation by HPLC,indicating that complex of gossypol with zinc was composed of five compounds.Among them, two were formed by the chelation of gossypol with Zn2+ at the molar ratio by 1∶2,and the other three were formed by the chelation of gossypol with Zn2+ at the molar ratio by 1∶1,1∶3 and 1∶4,respectively.The results of the study can well explain the phenomenon that the diet containing excessive free gossypol leads to the deficiency of zinc among mono-gastric animals, and causes a series of clinical symptoms of zinc deficiency.