声触诊组织成像定量技术在野百合碱诱导肝窦阻塞综合征大鼠模型中的无创性评价

Noninvasive evaluation of quantitative technique of acoustic touch tissue imaging in rat model of hepatic sinusoidal obstruction syndrome induced by monocrotaline

目的探讨声触诊组织成像定量(VTIQ)技术在野百合碱诱导的肝窦阻塞综合征大鼠模型中的无创性评价。方法SD大鼠70只随机分为对照组(n=10)和实验组(n=60)。对照组大鼠给予2 ml自来水灌胃;实验组大鼠给予浓度为130 mg/kg野百合碱2 ml单次灌胃,其中单纯灌胃组(MCT组,n=30)大鼠只给予野百合碱灌胃,灌胃加低分子肝素治疗组(MCT-L组,n=30)大鼠野百合碱灌胃24 h后皮下注射低分子肝素(96 iu,bid)。所有大鼠于灌胃后3、5 d行常规超声检查,检测大鼠门静脉内径及血流速度变化,然后应用VTIQ技术测量肝脏剪切波速度(SWV)值。VTIQ检查结束后2 h内,MCT组和MCT-L组分别处死15只大鼠,取肝脏标本行病理检查,光镜下参照Deleve评分标准判定病变。结果大鼠野百合碱单次灌胃后,体重下降。灌胃后3、5 d,与对照组相比,MCT组、MCT-L组的大鼠的门静脉内径无明显变化(P>0.05),门静脉流速降低、肝SWV值明显升高,差异均有统计学意义(P<0.05)。灌胃后3、5 d,与MCT组相比,MCT-L组大鼠门静脉内径未发生明显变化(P>0.05),门静脉流速有所升高、肝SWV值下降、肝组织学评分降低,差异均有统计学意义(P<0.05)。结论VTIQ技术检测肝脏的SWV,能反映肝脏组织病理学变化,可以无创性评价低分子肝素治疗野百合碱诱导的肝窦阻塞综合征的疗效。

Objective To investigate the noninvasive evaluation of virtual touch tissue imaging quantification(VTIQ)in the rat model of hepatic sinusoidal obstruction syndrome(HSOS)induced by monocrotaline.Methods A total of 70 SD rats were randomly divided into control group(n=10)and experimental group(n=60).Rats in the control group were given 2 ml tap-water by gavage.Rats in the experimental group were given 2 ml monocrotaline(130 mg/kg)by gavage.Rats in the MCT group(n=30)were only given monocrotaline by gavage.Rats in the MCT-L group(n=30)were given low molecular weight heparin(96 iu,bid)by subcutaneous injection 24 hours after monocrotaline gavage.The portal vein diameter and blood flow velocity were detected by conventional ultrasonography on the 3 rd and 5 th day,and then the shear wave velocity(SWV)of liver was measured by VTIQ.Within 2 hours after the VTIQ,15 rats in MCT group and 15 rats in MCT-L group were killed.Liver samples were taken for pathological examination,and the lesions were determined according to the Deleve grading standard under light microscopy.Results The body weight of rats decreased after a single intragastric administration of monocrotaline.Compared with the control group,the inner diameter of portal vein in MCT group and MCT-L group had no significant differences after 3 and 5 days′gavage(P>0.05).The portal vein velocity decreased,the liver SWV value increased and the liver histological score increased significantly(P<0.05).After 3 and 5 days′gavage,there was no significant difference in the diameter of portal vein between MCT-L group and MCT group(P>0.05),but the portal vein flow rate was significantly higher,liver SWV value and liver histological score were significantly lower in MCT-L group(P<0.05).Conclusion VTIQ could be used to detect SWV of liver,which could reflect the pathological changes of liver tissue and evaluate the therapeutic effect of low molecular weight heparin in the treatment of monocrotaline induced HSOS noninvasively.