合肥市2014—2019年副溶血性弧菌临床分离株病原学特征及耐药研究

Pathogenic characteristics and drug resistance of clinical isolates of vibrio parahaemolyticus in Hefei City from 2014 to 2019

目的了解2014—2019年合肥市副溶血性弧菌临床分离株所属的血清群、毒力基因和耐药特征,为副溶血性弧菌感染的预防和控制提供科学依据。方法采用玻片凝集法对107株副溶血性弧菌进行血清分群,Real-time PCR方法检测tlh、tdh和trh毒力基因,K-B琼脂纸片扩散法进行药敏试验。结果 107株副溶血性弧菌分属6个血清群,以O3群为主,占83.2%(89/107);其次为O4群,占13.1%(14/107)。tlh、tdh和trh毒力基因检出率分别为100%(107/107)、99.1%(106/107)和0(0/107)。107株副溶血性弧菌对氨苄西林和头孢唑啉的耐药率较高,分别达到84.1%(90/107)和82.2%(88/107)。对头孢西丁、头孢他啶和头孢吡肟耐药率为0.9%(1/107),对哌拉西林、诺氟沙星、左氧氟沙星、阿米卡星、庆大霉素、妥布霉素、甲氧苄啶/磺胺异恶唑、四环素、氯霉素和呋喃妥因完全敏感。结论近几年来,合肥市副溶血性弧菌临床分离株以O3群为主,tdh毒力基因携带率非常高,已有菌株耐药情况存在,临床应规范用药。

Objective To investigate the serogroups,virulence genes and drug resistances characteristics of vibro parahaemolyticus in Hefei City from 2014 to 2019.So as to provide scientific basis for the prevention and control of vibrio parahaemolyticus infection.Methods 107 strains of vibro parahaemolyticus were grouped by slide agglutination method,.TLH,TDH and TRH virulence genes were detected by real-time PCR.And drug sensitivity was tested by K-B method.Results The 107 strains of vibrio parahaemolyticus belonged to 6 serogroups.Group O3 was the main serogroup,accounting for 83.2%(89/107),followed by group O4,accounting for 13.1%(14/107).The detection rates of TLH,TDH and TRH virulence genes were 100%(107/107),99.1%(106/107)and 0(0/107),respectively.The resistance rates of 107 strains of vibrio parahaemolyticus to ampicillin and cefazolin were high,reaching 84.1%(90/107)and 82.2%(88/107),respectively.The drug resistance rate to cefoxitin,ceftazidime and cefepime was 0.9%(1/107),and it was completely sensitive to piperacillin,norfloxacin,levofloxacin,amikacin,gentamicin,tobramycin,trimethoprim/sulfaisoxazole,tetracycline,chloramphenicol and nitrofurantoin.Conclusion In recent years,the main clinical isolates of vibrio parahaemolyticus in Hefei City are O3 group,the TDH virulence gene carrying rate is very high,and the drug resistance of strains exists,so clinical medication should be standardized.