三倍体观赏百合与二倍体食用龙牙百合的杂交分析

Analysis of the Hybridization between Triploid Ornamental Lily(Lilium)and Diploid Edible Lilium brownii var.viridulum

为探讨异源三倍体百合与龙牙百合(BB)的杂交亲和性,实现观赏百合与食用百合的种质融合与创新,该研究以三倍体百合Triumphator(LLO)作母本,龙牙百合为父本,采用常规授粉与切柱头授粉,利用基因组荧光原位杂交(GISH)技术分析母本及子代的基因组成。结果显示:(1)通过常规授粉和切柱头授粉共获得17个发育良好的果实,通过胚抢救共获得了40株幼苗,且常规授粉的出苗率明显比切柱头授粉的高。(2)对随机选取的8个子代进行GISH分析,所鉴定的后代均为非整倍体,染色体数目为26~32条,其中东方百合基因组(O)染色体数目为2~8条,铁炮百合(L)与龙牙百合(B)基因组染色体始终为24条。(3)通过GISH技术无法区分铁炮百合与龙牙百合的染色体,亲本与子代均无重组现象。研究表明,龙牙百合与铁炮百合亲缘关系较近,龙牙百合作父本与三倍体百合Triumphator杂交可获得非整倍体,实现了观赏百合与食用百合的种质融合,为培育赏食兼用百合奠定了基础。

To explore the hybridization compatibility of triploid lily and Lilium brownii var.viridulum(BB),and realize the fusion and innovation of the ornamental and edible lily,we used triploid lily Triumphator(LLO)as female parent,L.brownii var.viridulum as male parents,and the normal and cut style pollination,and with genomic in situ hybridization(GISH)to analyze the genome composition of parents and progenies.The results showed that:(1)17 well-developed fruits were obtained,and 40 seedlings were obtained by embryo rescue,and the normal pollination was obviously better than that of cut style pollination.(2)GISH showed that all the progenies,which were selected randomly,were aneuploidy with 26-32 chromosomes,among which the O genome chromosome number were 2-8,and the sum of L and B genome chromosomes were 24 invariably.(3)Meaning O genome could not be distinguished from B genome using GISH,and there was no recombination in the parent and progenies.This illustrated,L.brownii var.viridulum and lilies would not be so distant,and when L.brownii var.viridulum as male parent was hybridized with the triploid Triumphator,aneuploidy lilies could be obtained.Since this way could realize the fusion and innovation of ornamental and edible lilies,it provide some valuable information to breed edible and ornamental lilies.