重组酶介导扩增技术及其在病原微生物快速检测中的应用进展

Research Advances in Recombinase-aided Amplification Technology and Its Application in Rapid Detection of Pathogenic Microorganisms

建立快速的病原学诊断方法对动物疫病的预防和控制、公共卫生安全等方面具有重要意义。重组酶介导扩增法(RAA)是一种新型恒温体外核酸扩增技术,在体外较低温度下就可以实现对DNA或RNA的快速扩增。RAA具有操作简便、快速、准确、节能、便捷等优点。重组酶、单链结合蛋白和DNA聚合酶是该技术的三大核心物质,利用这三种物质可替代传统PCR的热循环解链过程。该技术将会在病原微生物检测方面发挥重要作用。对RAA技术及其在病原检测方面的应用进行了综述,以期为相关领域的研究提供参考。

The establishment of rapid diagnostic methods for pathogens is of great significance for the prevention and control of animal epidemics and public health safety in China.Recombinase-aided amplification(RAA)is a novel isothermal amplification technology in which rapid amplification of DNA or RNA can be carried out at low temperatures,and the entire RAA reaction is simple,rapid,accurate,power-saving and convenient.There are three core substances of this technology,including recombinase,single-stranded DNA binding protein and DNA polymerase,which can replace the traditional PCR thermal cycle chain decomposition process.Therefore,the RAA technology will play a more and more important role in the detection of pathogenic microorganisms.The research advances in the detection of pathogens by the RAA technology was reviewed,which may provide reference for further relevant studies.