过表达miR-139-5p对胶质母细胞瘤U87MG细胞增殖、侵袭的影响

The effect and possible mechanism of miR-139-5p overexpression on the proliferation and invasion of glioma U87MG cells

目的探讨过表达miR-139-5p对胶质瘤U87MG细胞增殖和侵袭的影响,并初步分析其可能机制。方法在胶质瘤U87MG细胞中瞬时转染miR-139-5p并用real-time PCR方法验证其转染效率,CCK-8法检测转染后各组U87MG细胞的增殖能力,Transwell实验检测转染后各组U87MG细胞的侵袭能力,Western blot检测转染后各组U87MG细胞中BMI1蛋白的表达,应用生物信息网站Targetscan和双荧光素酶报告基因实验验证BMI1是否为miR-139-5p的靶基因。结果 miR-139-5p过表达使U87MG细胞增殖能力下降,侵袭能力下降,U87MG细胞中BMI1蛋白表达下降,生物信息学分析及双荧光素酶实验证实BMI1为miR-139-5p的靶基因。结论过表达miR-139-5p抑制U87MG细胞增殖及侵袭能力,与下调BMI1的表达相关。

Objective To investigate the effect and mechanism of overexpression of miR-139-5 p on the proliferation and invasion of glioma U87 MG cells. Methods Instantaneous transfection of miR-139-5 p mimics into glioma U87 MG cells was verified by real-time PCR. The proliferation and invasion of the were detected by CCK-8 kit and transwell respectively, Western blot was used to detect the expression of BMI1 protein, and the targetscan and double luciferase reporter gene experiment were used to verify whether BMI1 is the target gene of miR-139-5 p. Results Overexpression of miR-139-5 p inhibited the proliferation and invasion of glioma U87 MG cells, and the expression level of BMI1 protein was decreased. Bioinformatics and dual luciferase reporter gene confirmed that BMI1 was the target gene of miR-139-5 p. Conclusion miR-139-5 p inhibits the proliferation and invasion of glioma U87 MG cells via targeting BMI1.