ARID1A及PIK3CA在膀胱尿路上皮癌中的表达及临床病理意义

Expression and significance of ARID1A and PIK3CA in bladder urothelial carcinoma

目的:探讨膀胱尿路上皮癌(BUC)组织中ARID1A及PIK3CA的表达及意义。方法:采用免疫组化EnVision法检测107例膀胱尿路上皮癌(BUC组)及28例癌旁组织(癌旁组)中ARID1A、PIK3CA及Ki-67的表达水平,并将BUC组按照临床病理特征进行分组,统计分析ARID1A、PIK3CA在各组间表达的差异及相关性。结果:ARID1A在BUC组中有42.06%呈缺失表达,而在癌旁组中仅7.14%呈缺失表达,二者差异有统计学意义(P<0.05)。PIK3CA在BUC组中有74例(69.16%)表达显著增强,而在癌旁组中仅7例(25%)呈强阳性表达,两组间比较差异有统计学意义(P<0.05)。结合患者的临床病理特征进行分析,发现ARID1A的缺失表达与肿瘤的浸润深度、分级及Ki-67增殖指数有关(P<0.05),PIK3CA的阳性表达与肿瘤的浸润深度有关(P<0.05),与分级、Ki-67增殖指数无关(P>0.05)。相关性分析结果显示ARID1A与PIK3CA之间的表达无明确相关性(r=-0.062,P>0.05),复发与否与年龄、病变级别、浸润深度、PIK3CA蛋白阳性表达率呈正相关。结论:ARID1A及PIK3CA在BUC患者癌组织中的表达明显增强,且与病变级别、肿瘤浸润密切相关,但两种蛋白间并无相关性,二者可能通过不同的途径参与BUC的发生、进展。并且PIK3CA蛋白阳性表达可明显增加BUC的复发率,进一步为探究BUC提供了新的思路。

Objective:To investigate the expression and significance of ARID1 A and PIK3 CA in bladder urothelial carcinoma(BUC).Methods:Immunohistochemical methods were employed to evaluate protein level of ARID1 A and PIK3 CA in 107 cases of BUC and 28 cases of adjacent tissues.The BUC tissues were grouped by clinical and pathological features,and the difference and relations of ARID1 A and PIK3 CA in each group were analyzed.Results:The loss rate of ARID1 A was 42.06%in BUC,but only 7.14%in adjacent tissues.The positive rate of PIK3 CA in BUC(69.16%)was significantly higher than that in adjacent tissues(25%)(P<0.05).The clinical features of 107 cases of BUC were retrospectively analyzed,which showed that the expression of ARID1 A was significantly associated with histopathology grading,depth of invasion and Ki-67 index.The expression of PIK3 CA was significantly associated with depth of invasion,but not related to age,sexuality,recurrence or Ki-67 index(P>0.05).The results of correlation analysis showed that there was no clear correlation between ARID1 A and PIK3 CA(r=-0.062,P>0.05).The recurrence was positively correlated with age,histopathology grading,depth of invasion and PIK3 CA.Conclusion:ARID1 A and PIK3 CA play an important role in the development and progression of BUC through different pathways.The expression of PIK3 CA maybe increase the recurrence of BUC.It will provide a new idea for exploring the prevention and treatment of urothelial carcinoma of the bladder.