低温弱光下aga2突变体西瓜果实糖代谢差异表达基因分析

Analysis of Differentially Expressed Genes of Sugar Metabolism in aga2 Mutant Watermelon Fruit under Low Temperature and Weak Light

为了探明低温弱光环境对西瓜果实糖分积累的调节机制,本试验通过分析棉籽糖水解酶碱性α-半乳糖苷酶基因aga2突变体转录组数据发现低温弱光下aga2突变体西瓜果实中有686个基因上调表达,990个基因下调表达,进一步通过GO和KEGG分析发现MYB、NAC、WRKY等转录因子上调表达抵御低温弱光;而肌醇半乳糖苷合酶(Cla009222)、α-半乳糖苷酶(Cla022883、Cla019238)等与糖分代谢相关的基因在低温弱光下下调表达可能是导致aga2突变体果实糖含量下降的主要原因。本试验通过分析转录组数据,挖掘响应低温弱光的西瓜糖分积累相关基因,为西瓜果实响应低温弱光的糖代谢调控网络奠定了基础。

In order to explore the regulatory mechanism of low temperature and weak light environment on sugar accumulation of watermelon,this experiment analyzed the transcriptome data of mutant of alkaline alpha-galactosidase gene aga2,and found that there were 686 up-regulated genes and 990 down-regulated genes in aga2 mutant fruits under low temperature and weak light.By GO and KEGG analysis,it was further found that the up-regulated expression of transcription factors:MYB,NAC and WRKY etc.could resist low temperature and weak light.While,the down-regulated expression of genes related to sugar metabolism like galactinol synthase(Cla009222),alpha-galactosidase(Cla022883、Cla019238)etc.induced by low temperature and weak light may be the main reason for the decrease of sugar content in mutant fruit.By analyzing the transcriptome data,this experiment excavated the genes related to sugar accumulation in watermelon responding to low temperature and weak light,and laid the foundation for regulation network of sugar metabolism in watermelon responding to low temperature and weak light.