乙烯代谢和能量状态对‘巴茨拉’牡丹切花瓶插品质的作用研究

Effects of Ethylene Metabolism and Energy Status on Vase Quality of Cut Itoh Peony’Bartzella’Flowers

以发育至绽口期的‘巴茨拉’牡丹切花为试材,分别经蒸馏水和20 mg·L^(-1)纳米银预处理2 h,再进行蒸馏水或保鲜液瓶插,测定瓶插过程中花瓣乙烯释放速率,1-氨基环丙烷-1-羧酸(ACC)、腺苷酸(ANP)和糖含量,乙烯和糖代谢相关酶活性。结果表明,纳米银预处理乙烯释放峰值降幅达40.9%,降低了ACC含量和ACC合成酶(ACS)活性,提高了琥珀酸脱氢酶(SDH)和H^(+)-ATP酶活性,增加了糖和ATP含量,保持较高的能荷(EC)水平,切花瓶插寿命延长了40.7h。保鲜液瓶插乙烯释放峰值降幅达35.1%,提高了SDH活性与糖及ATP含量,保持较高的EC值,最大花径增加了3.5 cm。纳米银预处理后保鲜液瓶插(NS+PS)乙烯释放峰值降幅达31.2%,降低了ACC含量和ACS活性,提高了SDH、CCO和H^(+)-ATP酶活性,糖及ANP含量,维持了较高的EC水平,提高了可溶性蛋白质含量,瓶插寿命延长了46.4 h,最大花径增大3.6 cm。结果显示通过抑制乙烯代谢和增强能量状态可延缓‘巴茨拉’牡丹切花衰老提高瓶插品质。

The regulatory effects of ethylene metabolism and energy status on the vase quality of cut Itoh peony’Bartzella’flowers were studied by using nano silver and preservative solution treatment.Cut Itoh peony flowers with a developmental level of stageⅡwere employed as test materials and were pretreated with distilled water(the control)or 20 mg·L^(-1) nano silver(NS)for 2 h.The samples were then vased in preservative solution(PS)directly after distilled water treatment or after nano silver treatment(NS+PS).The release rate of ethylene,the contents of 1-aminocyclopropane-1-carboxylic acid(ACC),adenine nucleotides(ATP+ADP+AMP),sugar,and the activities of enzymes related to ethylene and sugar metabolism during the vase insertion process were measured.The results demonstrated that nano-silver pretreatment(NS)inhibited the peak of ethylene release by 40.9%,and reduced ACC content and ACC synthetase(ACS)activity,while increased the activities of succinic dehydrogenase(SDH)and H^(+)-ATPase,and contents of sugar and ATP,which maintained a high energy charge(EC)level,therefore,prolonged vase life by 40.7 h.The PS also inhibited the peak of ethylene release by 35.1%,and increased SDH activity and sugar and ATP contents,which maintained a high EC value,and increased the maximum flower diameter by 3.5 cm.Moreover,NS+PS group showed inhibition of ethylene release peak by 31.2%,and reduction of ACC content and ACS activity,while SDH,CCO,and H^(+)-ATPase activities,and the contents of sugar and ATP were all increased,which also maintained high EC levels and increased the soluble protein content,and therefore,increased flower vase life by 46.4 h,and the maximum flower diameter by 3.6 cm.The results showed that inhibition of ethylene metabolism and enhancement of energy status could delay the senescence and improve the vase quality of Itoh peony’Bartzella’cut flowers.