黄孢原毛平革菌产漆酶优化培养及其对刚果红的脱色降解

Optimization of culture conditions for laccase production of Phanerochaete chrysosporium and efficiency of the laccase in Congo Red degradation

以白腐真菌模式菌株黄孢原毛平革菌Phanerochaete chrysosporium为研究对象,探讨培养条件、重金属和芳香族化合物对产漆酶的影响,并进一步研究漆酶对刚果红的降解效果。结果表明,P.chrysosporium产漆酶最适培养条件:葡萄糖为碳源,蛋白胨为氮源,碳氮比为90。培养8d后,漆酶酶活为911.1U/L;Mn^(2+)严格地控制着P.chrysosporium产漆酶,而Cu^(2+)对其影响不大,在添加4.0mmol/L Mn2+时,漆酶酶活为2 001.7U/L;芳香族化合物中藜芦醇(veratryl alcohol,VA)、4-香豆酸、香草醛和香草酸对菌体产漆酶能力均有促进作用,最高可提升至1 459.1U/L,而咖啡酸对菌体产漆酶稍有抑制。100U/L漆酶粗酶液可降解40mg/L刚果红,降解率为24.0%;而当介体物质VA存在时,该降解效率可提升至87.7%。

The effects of culture conditions, heavy metals and aromatic compounds on laccase production by the model strain of Phanerochaete chrysosporium were investigated, and degradation efficiency of the crude laccase to Congo Red was further detected. The results showed that the optimum medium for laccase production were glucose as carbon source and peptone as nitrogen source in the carbon-to-nitrogen ratio of 90. After 8 d of culture, laccase activity was 911.1 U/L. Mn2+ strictly controlled the laccase production by P. chrysosporium, while Cu^(2+) had little influence. When 4.0 mmol/L Mn^(2+) was added, the laccase activity was 2 001.7 U/L. The laccase production could be promoted by VA, 4-coumaric acid, vanillin aldehyde and vanillin acid, reaching the highest level of 1 459.1 U/L, while coffee acid inhibited the laccase production slightly. 100 U/L crude laccase could degrade 40 mg/L Congo Red with degradation efficiency of 24.0%, and the degradation efficiency could increase to 87.7% by using VA as the redox mediator.