北京市顺义区腹泻病例分离沙门氏菌耐药特征分析

Drug resistance characteristics of Salmonella isolated from diarrheal patients in Shunyi, Beijing

目的对北京市顺义区腹泻病例分离的沙门氏菌进行耐药特征分析。方法 2个收集2013—2018年北京市顺义区腹泻病例粪便标本,对标本进行沙门氏菌分离培养、生化鉴定和血清分型,使用微量肉汤法对分离的沙门氏菌菌株进行26种抗菌药物的敏感性检测,开展耐药率、非敏感率、多重耐药率和耐药谱分析。结果腹泻病例沙门氏菌检出率为5.44%(113/2 076),包含26种血清型,其中肠炎沙门氏菌构成比为32.46%(37/114),鼠伤寒沙门氏菌构成比为27.19%(31/114)。沙门氏菌多重耐药率为44.74%(51/114),分布62种耐药谱;肠炎沙门氏菌多重耐药率为67.57%(25/37),分布29种耐药谱。鼠伤寒沙门氏菌多重耐药率为45.16%(14/31),分布20种耐药谱。结论北京市顺义区分离自腹泻病例的沙门氏菌耐药状况较为严重,耐药谱分布呈现多样性。应持续开展沙门氏菌病原学监测,为沙门菌感染的科学防控和治疗提供依据。

Objective To study the drug resistance characteristics of Salmonella isolated from diarrheal patients in Shunyi, Beijing. Methods Collected stool samples of diarrheal patients in Shunyi, Beijing 2013-2018. Isolation, bacterial identification and serotyping were tested for Salmonella strains. Drug resistance rate, non-drug sensitivity rate, multi-drug resistance rate and drug resistance profile of Salmonella strains to 26 antibacterial drugs were analyzed by broth dilution test method. Results The prevalence of Salmonella in diarrhea patients was 5.44%(113/2 076). Twenty-six serotypes were identified in 114 Salmonella strains, and the dominate serotypes were Salmonella enteritidis and Salmonella typhimurium with the composition ratio value of 32.46%(37/114) and 27.19%(31/114), respectively. The multi-drug resistance rates of Salmonella spp., S. enteritidis and S.typhimurium were 44.74%(51/114), 67.57%(25/37) and 45.16%(14/31), respectively. Salmonella spp., S.enteritidis and S.typhimurium were distributed 62, 29 and 20 kinds of drug resistance profiles, respectively. Conclusion The drug resistance of Salmonella isolated from diarrhea patients was serious, and the distribution of drug resistance profile showed large diversity. It is necessary to continued high-quality pathogenic surveillance of Salmonella, so as to provide basis for scientific prevention and treatment of Salmonella infection.