缺血性脑卒中患者外周血中miR-488分子标志物实验研究

The experimental study of miR-488 molecular marker in peripheral blood of patients with ischemic stroke

目的研究miR-488在缺血性脑卒中患者血清中的表达变化,探讨其作为诊断标志物对缺血性脑卒中的诊断价值。方法选择2020年8月至2021年2月间于牡丹江医学院附属红旗医院就诊的缺血性脑卒中患者60例作为病例组(按发病时间分为3 d病例组和7 d病例组),收集同期来我院体检的健康体检者30例作为对照组,采用实时荧光定量聚合酶链反应(qRT-PCR)测定所有研究对象外周血血清中miR-488的表达水平;记录研究对象的血糖、血压、血脂等指标。结果病例组血清中miR-488相对表达量(3.37±1.16)高于对照组(1.91±0.82)(P<0.01);3 d病例组血清miR-488相对表达量(2.99±1.10)高于对照组(1.91±0.82)(P<0.01);7 d病例组血清miR-488相对表达量(3.75±1.11)高于对照组和3 d病例组(P<0.01);ROC曲线分析结果显示,AUC为0.839,敏感度为85%,特异度为70%。结论缺血性脑卒中患者血清中miR-488表达水平明显升高,对缺血性脑卒中早期(7d内)具有潜在的诊断价值。

Objective To investigate the expression of miR-488 in serum of patients with ischemic stroke,and to explore the diagnostic value of miR-488 as a diagnostic maker.Methods From August 2020 to February 2021,60 patients with ischemic stroke were enrolled as the case group(divided into 3 d case group and 7 d case group according to the time of onset)and the 30 healthy subjects who were treated in the Red Flag Hospital affiliated to the Mudanjiang Medical College as the control group.The quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression of miR-488 in peripheral blood serum of different groups.The indexes,including blood sugar,blood pressure and blood fat group and the control group,were recorded.Results The relative expression level of miR-488 in serum of case group(3.37±1.16)was higher than that in the control group(1.91±0.82)(P<0.01).The relative expression level of serum miR-488 in 3 d group(2.99±1.10)was higher than that in control group(1.91±0.82)(P<0.01).The relative expression level of serum miR-488 in the 7 d group(3.75±1.11)was higher than in the control group and the 3 d group(P<0.01).ROC curve showed that AUC was 0.839 and 95%CI was 0.756~0.921.The sensitivity was 85%and the specificity was 70%.Conclusion The level of miR-488 in serum of patients with ischemic stroke is significantly increased,which has a higher diagnostic value in the early stage(with in 7 days)of ischemic stroke.