MicroRNA-146a与CXCR4在糖尿病心肌病心肌成纤维细胞中的表达变化

To investigate the expression changes ofMicroRNA-146a and CXCR4 in myocardial tissue and fibroblasts of diabetic cardiomyopathy

目的:探究微小RNA-146a(miR-146a)与趋化因子受体4(CXCR4)在糖尿病心肌病心肌成纤维细胞(CFs)中的表达变化。方法:提取原代心肌成纤维细胞(Cardiac fibroblasts,CFs),高糖(33.3mmol·L^(-1))作用CFs。用qRT-PCR检测CFs中miR-146a的表达,Western blotting检测CFs中α-平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原前胶原A1(Col1A1)、CXCR4蛋白的表达情况。MTT检测高糖对CFs增殖活性的影响。结果:与正常对照组相比,高糖诱导后的CFs中miR-146a表达量降低,而高糖诱导后的CFs中α-SMA、Col1A1、CXCR4表达增加。并且高糖处理后CFs增殖活性提高。结论:糖尿病心肌病形成过程中CFs中miR-146a表达下调,而CXCR4表达上调,提示miR-146a与CXCR4糖尿病心肌病的病程中可能存在潜在的调节关系,有助于探索糖尿病心肌病的病理机制。

Objective:To investigate the expression changes of microRNA-146a(miR-146a)and chemokine receptor 4(CXCR4)in diabetic cardiomyopathy myocardial fibroblasts(CFS).Methods:CFS were extracted and high glucose(33.3mmol·L^(-1))was used to induce CFs.Use qRT-PCR in the detection of CFs miR-146a express,Western blotting detection in CFs the expression ofα-SMA,Col1A1,and CXCR4.MTT was used to detect the effect of high glucose on the proliferation of CFS.Results:Compared with the normal control group,the expression of miR-146a decreased in CFs induced by high glucose,while the expression ofα-SMA,Col1A1 and CXCR4 increased in CFs induced by high glucose.The proliferation activity of CFs was increased after high glucose treatment.Conclusion:During the formation of diabetic cardiomyopathy,miR-146a expression is down-regulated in CFs,while CXCR4 expression is up-regulated,suggesting that there may be a potential regulatory relationship between miR-146a and CXCR4 in the course of diabetic cardiomyopathy,which is helpful to explore the pathological mechanism of diabetic cardiomyopathy.