1,8-桉叶素驱蚊止痒功效的研究

Study on the mosquito-repelling and antipruritic effects of 1,8-cineole

为探究1,8-桉叶素驱蚊及其治疗局部皮肤过敏性瘙痒的效果,通过"Y"型嗅觉仪法测试了其在不同剂量梯度下对白纹伊蚊的驱避活性,采用4-氨基吡啶(4-AP)致小鼠瘙痒模型探讨了1,8-桉叶素(φ=5%,用橄榄油稀释)的止痒作用。结果表明,当1,8-桉叶素剂量为4.0μL时,其在前4 min内对白纹伊蚊可达到100%的驱避率,且其对白纹伊蚊的驱避效果呈剂量依赖性增加;通过4-AP致小鼠瘙痒模型发现,与模型组相比,1,8-桉叶素组小鼠在10 min内的舔体次数显著减少,小鼠皮肤滤液中组胺与血清中白细胞介素31(IL-31)、血小板活化因子(PAF)、5-羟色胺(5-HT)含量均显著降低,病理切片显示1,8-桉叶素组小鼠皮肤未见水肿,同时炎细胞浸润减少。结论:当1,8-桉叶素浓度达到一定值时,其对白纹伊蚊具有很好的驱避效果;且1,8-桉叶素具有显著的止痒效果,其发挥止痒作用与拮抗IL-31、PAF、5-HT、瘙痒介质组胺的释放有关。

To explore the mosquito repellent effect and the therapeutic properties of allergic skin itching of 1,8-cineole,the repellent activity of 1,8-cineole against Aedes albopictus under different doses was studied by the Y-tube olfactory test.The antipruritic effect of 1,8-cineole(φ=5%,prepared with olive oil)was studied in an animal model of mice skin scratching response induced by 4-aminopyridine(4-AP).The results show that 1,8-cineole at the dose of 4.0μL repels 100%of A.albopictus in the initial 4 minutes and the repellent effect of 1,8-cineole against A.albopictus increases in a dose-dependent manner.In addition,mice experiments show that the scratching frequency of mice treated with 1,8-cineole reduces significantly within 10 minutes;and the contents of serum interleukin 31(IL-31),platelet activating factor(PAF),serotonin(5-HT)and histamine in the skin filtrate of mice treated with 1,8-cineole are significantly reduced compared with the model group.The pathological sections show that no edema in the skin of mice in the 1,8-cineole group is observed and the infiltration of inflammatory cells is inhibited.In conclusion,when the concentration of 1,8-cineole reaches a certain value,it shows obvious repellent effect on A.albopictus.1,8-cineole also has a significant antipruritic effect through the mechanism of antagonizing the release of IL-31,PAF,5-HT,as well as the mediator of itching,histamine.