不同种类甘草成分及美白抗敏活性差异研究

anti-allergy activities of different kinds of licorice

以甘草的3种不同基源植物胀果甘草、光果甘草及甘草为研究对象,比较其成分及活性差异。采用高效液相色谱法测定甘草苷、甘草酸及光甘草定的含量,并进行比较;通过紫外分光法比较3种甘草的总黄酮含量差异,并对酪氨酸酶及透明质酸酶活性抑制进行比较。结果显示,高效液相色谱法的精密度、稳定性、重复性、加样回收率的RSD值均不大于2.09%;含量分析结果表明,甘草酸及光甘草定在光果甘草中含量最高,甘草苷在甘草含量中最高。甘草药材总黄酮含量大小顺序为:光果甘草>甘草>胀果甘草;对酪氨酸酶抑制率大小顺序为:光果甘草>甘草>胀果甘草,IC_(50)值分别为7.36,8.38和11.13 mg/L;对透明质酸酶抑制率大小顺序为:胀果甘草>甘草>光果甘草,IC_(50)值分别为8.70,9.19和10.45 mg/mL。3种甘草对酪氨酸酶及透明质酸酶抑制良好,可能具有较好的美白和抗敏功效,为了达到最佳效果需要区分使用。

Three different plants of licorice,G.inflata,G.glabra and G.uralensis,were used as the research objects to compare the differences in the composition and activities.The content of liquiritin,glycyrrhizic acid and glabridin was determined by HPLC-DAD;the difference in total flavonoid content was compared by UV spectrophotometry,and the inhibition of tyrosinase and hyaluronidase activities was compared.The target compounds were extracted with 70%ethanol,and separated on an Agilent Poroshell 120 EC-C18(150 mm×4.6 mm,2.7μm)in gradient elution program using methanol and 0.05%phosphoric acid as mobile phase,at a flow rate of 1 mL/min at 30℃,and using a detection wavelength of 270 nm.The validated HPLC method shows good linear relationship with correlation coefficients larger than 0.99885.The relative standard deviation(RSD)values of precision,stability and repeatability are less than 0.89%.The average recoveries are in the range of 97.50%-99.60%with the RSD values less than 2.09%.The analysis results show that the content of glycyrrhizic acid and glabridin are the highest in G.glabra,and the content of liquiritin is the highest in G.uralensis;the total flavonoid content of licorice ranks in a sequence that G.glabra>G.uralensis>G.inflata;The sequence of inhibition rate of tyrosinase is G.glabra>G.uralensis>G.inflate,and their IC50 values are 7.36,8.38 and 11.13 mg/L,respectively;the sequence of inhibition rate of hyaluronidase is G.inflata>G.uralensis>G.glabra,and their IC50 values are 8.70,9.19 and 10.45 mg/mL,respectively.The three kinds of licorice may have good whitening and anti-allergy effects because of their good inhibition of tyrosinase and hyaluronidase.To achieve the best effect,they need to be used separately.