摘要
The reactive oxygen species(ROS) are tightly associated with endoplasmic reticulum(ER) stress.Thus,the deep and visual insight of aberrant ROS fluctuations in the ER can help us better investigate the ER stress-associated pathology.In this work,a fluorescent probe ERC for HOCI detection in the ER based on phenothiazine-derived coumarin platform was developed.In the presence of HOCI,ERC exhibited an emission change from 609 nm to 503 nm within seconds.It also showed high sensitivity(0.44 μmol/L)and superb photostability.Significantly,ERC displayed low cytotoxicity,good cell membrane permeability,and appreciable ER-targetability.Ultimately,the probe was successfully utilized to image exogenous and endogenous HOCl in living cells and reveal the HOCI burst in cisplatin-treated cancer cells.
基金
This work was supported by the Hubei Provincial Department of Education Science and Technology Research Projects(No.Q20182704).
