膜联蛋白A7过表达对人胃癌MGC-803细胞增殖和凋亡的影响

Effects of ANXA7 Overexpression on Proliferation and Apoptosis of Human Gastric Cancer MGC-803 Cells

目的探讨膜联蛋白A7(ANXA7)过表达对人胃癌MGC-803细胞增殖和凋亡的影响。方法将人胃癌MGC-803细胞分为ANXA7过表达组、阴性对照组,脂质体转染法将pcDNA3.1-ANXA7重组质粒以及空质粒分别转染ANXA7过表达组和阴性对照组。采用Western blot鉴定ANXA7过表达;转染48h采用CCK-8实验检测各组细胞增殖能力;流式细胞术检测各组细胞凋亡率;Western blot检测各组细胞增殖和凋亡相关蛋白PCNA、CyclinD1、Casepase-3、Cleaved casepase-3的表达。结果与阴性对照组相比,ANXA7过表达组MGC-803细胞增殖能力显著增强、PCNA与CyclinD1的表达均升高(P<0.05);细胞凋亡率降低、Cleaved casepase-3的表达降低(P<0.05);Casepase-3的表达变化无统计学意义(P>0.05)。结论ANXA7过表达可促进人胃癌MGC-803细胞的增殖并抑制其凋亡。

Objective To investigate the effects of annexinA7(ANXA7)overexpression on the proliferation and apoptosis of human gastric cancer MGC-803 cells.Methods Human gastric cancer MGC-803 cells were divided into ANXA7 overexpression group and negative control group.PcDNA3.1-ANXA7 recombinant plasmid and empty plasmid were transfected into ANXA7 overexpression group and negative control group respectively by liposome transfection.The overexpression of ANXA7 was detected by Western blot.CCK-8 assay was used to detect the proliferation ability of cells in each group.Cell apoptosis rate was detected by flow cytometry,and the expressions of PCNA,CyclinD1,Casepase-3 and Cleaved Casepase-3 were detected by Western blot.Results Compared with the negative control group,the proliferation ability of MGC-803 cells in the ANXA7 overexpression group was significantly enhanced,and the expression of PCNA and CyclinD1 were both increased(P<0.05).Apoptosis rate and Cleaved casepase-3 expression were decreased(P<0.05).The expression of Casepase-3 was not statistically significant(P>0.05).Conclusion The overexpression of ANXA7 can promote the proliferation and inhibit the apoptosis of human gastric cancer MGC-803 cells.