肺泡灌洗液多重PCR检测对儿童社区获得性肺炎病原学的诊断价值

Value of multiplex PCR of bronchoalveolar lavage fluid in pathogen diagnosis of community-acquired pneumonia in children

目的:探讨肺泡灌洗液多重PCR检测对儿童社区获得性肺炎病原学的诊断价值。方法:选择2018年6月1日至11月30日在我院呼吸科住院的社区获得性肺炎患儿52例为研究对象,比较痰培养、肺泡灌洗液培养、肺泡灌洗液多重PCR 3种方法的病原体检出情况。结果:肺泡灌洗液多重PCR的单种病原体阳性检出率、两种及以上病原体混合感染检出率均显著高于其他两种方法(P<0.001);肺泡灌洗液多重PCR与血清ELISA的肺炎支原体及衣原体抗体阳性检出率差异无统计学意义(P>0.05)。结论:肺泡灌洗液多重PCR检测对社区获得性肺炎患儿病原体的检出率较高,在病原体混合感染检测方面更具优势。

Objective:To investigate the value of multiplex PCR of bronchoalveolar lavage fluid(BALF)in the pathogen diagnosis of community-acquired pneumonia(CAP)in children.Methods:52 children with CAP admitted to the Department of Respiratory Medicine of our hospital from June 1 to November 30,2018 were selected to compare the detection results of pathogens by sputum culture,BALF culture and multiplex PCR of BALF.Results:The positive rates of single bacteria and mixed infection of two or more pathogens in BALF by multiplex PCR were significantly higher than those by the other two methods(P<0.001);there was no significant difference in the positive rates of mycoplasma and chlamydia antibodies between multiplex PCR of BALF and serum ELISA(P>0.05).Conclusion:Multiplex PCR of BALF has a high detection rate for pathogens in children with CAP,and has more advantages in the detection of pathogen mixed infection.