杨树桑黄多糖提取工艺与测定

Extraction Technology and Determination of Polysaccharide from Sanghuangporus vaninii

通过比较微波提取、超声提取、加热回流提取、70℃水浴浸提4种方式对桑黄子实体中多糖工艺的提取,参考多糖含量以及遵循科学环保的原则选定最佳提取方法,以响应面法优化多糖提取工艺。比较苯酚-硫酸法与蒽酮-硫酸法测定多糖含量。结果表明,超声提取法可用于杨树桑黄的多糖提取,响应面法优化的最佳提取条件为:超声时间25 min、液料比400∶1(mL/g)、超声功率60 kHz;蒽酮-硫酸法和苯酚-硫酸法均可用于杨树桑黄中多糖含量的测定。基于"多糖"测定以及实验的稳定性,证明苯酚-硫酸法更适合测定杨树桑黄中多糖的含量,该结果可为其建立质量控制标准作参考。

The work preliminarily screened the methods for the extraction of polysaccharides from Sanghuangporus vaninii fruiting bodies combining technologies including microwave extraction, ultrasonic extraction, heating reflux extraction, and 70 ℃ water bath.The optimal extraction methods were selected based on the content and environment-friendliness factors by response surface analysis.Phenol-sulfuric acid and the anthrone-sulfuric acid methods were used to determine the polysaccharide content.The results showed that the optimum conditions for ultrasonic extraction method was as follows: ultrasonic time 25 min, liquid-to-material ratio 400∶1(mL/g),ultrasonic power of 60 kHz.Both the anthrone-sulfuric acid and phenol-sulfuric acid methods were suitable for the determination of the content of polysaccharides in Sanghuangporus vaninii.However, the phenol-sulfuric acid method was preferred due to the durability of the experiment.These results could be used as a reliable reference to establish quality control standards of polysaccharides from Sanghuangporus vaninii.