体外模拟消化对酸枣仁蛋白酶解产物抗氧化活性的影响

Effect of simulated gastrointestinal digestion in vitro on the antioxidant activity of Semen ziziphi spinosae protein hydrolysates

目的:以具有良好抗氧化活性的酸枣仁蛋白酶解产物(SZPHs)为研究对象,探究体外模拟胃肠消化对其氨基酸组成及抗氧化活性的影响。方法:通过体外模拟胃肠消化,得到酸枣仁蛋白酶解产物消化物(SZPHs-GD),并分析SZPHs消化前后的氨基酸组成以及体外抗氧化活性。结果:体外模拟胃肠消化后,SZPHs-GD中疏水性氨基酸含量降低了1.51%,亲水性氨基酸和带负电荷氨基酸含量分别升高了0.83%和2.11%,必需氨基酸含量无显著变化;分子量分布结果表明,SZPHs及SZPHs-GD分子量主要分布在5 kDa以下,且体外模拟消化后<5 kDa组分比例增加;经体外模拟胃肠消化后,SZPHs-GD对DPPH自由基清除率最高可达81.25%,对ABTS+自由基清除率最高可达90.40%,对超氧阴离子自由基清除率最高可达47.51%,对羟基自由基清除率最高可达32.66%。结论:经模拟体外胃肠消化后,SZPHs-GD仍具有较好的氨基酸组成以及较高的体外抗氧化活性。

Objective:In this study,semen ziziphi spinosae protein hydrolysates(SZPHs)with good antioxidant activity were used as the research object.Methods:By simulating gastrointestinal digestion in vitro,the semen ziziphi spinosae protein hydrolysates gastrointensive digest(SZPHs-GD)was obtained.The amino acid composition and antioxidant activity of SZPHs before and after digestion were analyzed in order to explore the effect of internal environment on the structure and activity of SZPHs.Results:The results showed that after simulated digestion in vitro,the content of hydrophobic amino acids in SZPHs-GD decreased by 1.51%,the content of hydrophilic amino acids and negatively charged amino acids increased by 0.83%and 2.11%,respectively,and the content of essential amino acids had no significant change;the results of molecular weight distribution showed that the molecular weight of szphs and szphs GD were mainly below 5 kDa,and the proportion of<5 kDa components increased after simulated digestion;the results of antioxidant activity test show that,after simulated gastrointestinal digestion in vitro,the highest DPPH radicals scavenging rate of SZPHs-GD was 81.25%,ABTS+radicals scavenging rate was 90.40%,superoxide radicals scavenging rate was 47.51%,hydroxyl radicals scavenging rate was 32.66%.Conclusion:SZPHs-GD still had good amino acid composition and antioxidant activity after simulating gastrointestinal digestion.