脉冲强光对饮料瓶盖杀菌效果

Effect of pulsed light on bacterium sterilization of beverage bottle cap

目的:研究脉冲强光杀菌技术对饮料瓶盖上芽孢杆菌的杀灭效果,拓展其在PET无菌灌装中的应用前景。方法:以初始菌落数、脉冲照射距离、脉冲闪烁次数为影响因子,探究脉冲强光杀菌技术对38 mm及28 mm饮料瓶盖上芽孢杆菌的灭菌效果。结果:初始菌落数越高、照射距离越短、闪烁次数越多,杀菌效率越高,且对38 mm瓶盖的杀菌效率高于28 mm瓶盖的。脉冲照射距离为7 cm,闪烁次数5次时,脉冲强光对38 mm瓶盖的最高杀菌效率为4.87 log,对28 mm瓶盖的最高杀菌效率为4.71 log,最高可将初始菌落数为1×10^(2)CFU的瓶盖上芽孢杆菌完全杀灭;照射距离为7 cm,闪烁次数为10次时,脉冲强光对38 mm瓶盖最高杀菌效率为5.34 log,对28 mm瓶盖的最高杀菌效率为5.28 log,最高可将初始菌落数1×10^(3)CFU的瓶盖上芽孢杆菌完全杀灭。结论:脉冲强光杀菌技术对饮料瓶盖上芽孢杆菌具有较强的杀灭作用,可用于饮料无菌灌装过程中HDPE瓶盖的杀菌。

Objective:This study aimed to study the effect of pulsed light on sterilization of beverage bottle caps and to expand its application in PET aseptic filling.Methods:The effect of pulsed light(PL)on the sterilization of 38 mm and 28 mm beverage bottle caps was evaluated.The factors affecting sterilization efficiency included initial aerobic bacterial count,PL irradiation distance and pulse frequency.Results:The results showed that the sterilization efficiency was proportional to the initial aerobic bacterial count and flash time,while it was inversely proportional to PL irradiation distance.When the PL treatment was performed by 7 cm irradiation distance and 5 flash times,the maximum sterilization efficiencies for 38 mm and 28 mm bottle caps were 4.87 log and 4.71 log,respectively.Meanwhile,the bacillus on bottle caps were entirely killed with the initial aerobic bacterial counts of 1×10^(2)CFU.While the PL treatment was conducted by 7 cm irradiation distance and ten flash times,the maximum sterilization efficiencies for 38 mm and 28 mm bottle caps were 5.34 log and 5.28 log,respectively.Moreover,the bacillus on bottle caps was killed completely with the initial aerobic bacterial counts of 1×10^(3)CFU.Conclusion:The pulsed light sterilization technology could kill the bacillus on the bottle caps to a certain extent and be applied to sterilizing HDPE bottle caps in the aseptic filling process of beverage products.