摘要
本文以‘翠香’猕猴桃带腋芽茎段为外植体,研究了不同激素配比对‘翠香’猕猴桃茎段腋芽诱导、增殖培养、生根培养的影响,并进行移栽。研究表明:‘翠香’猕猴桃最优的茎段腋芽诱导培养基为MS+2.0 mg·L^(-1)6-BA+0.3mg·L^(-1)NAA,诱导率为88.89%;最优的增殖培养培养基为MS+5 mg·L^(-1)6-BA+0.4 mg·L^(-1) NAA,增殖系数达6.20,三代连续增殖稳定;生根培养的最佳培养基为1/2MS+0.9 mg·L^(-1) IBA,生根率为94.44%,根数为11.40,根长为2.94;炼苗后移栽成活率达88.33%。此研究为‘翠香’猕猴桃的组织培养工厂化育苗提供了技术支持。
The stems with axillary buds of Actinidia chinensis var.deliciosa’cuixiang’was used as explants to study the effects of combinations of different plant growth regulator on induction of axillary buds,multiplication culture,rooting culture,and of transplanting.The most appropriate medium of induction of axillary buds was MS+2.0 mg·L^(-1)6-BA+0.3 mg·L^(-1) NAA,and a maximum induction rate was 88.89%.The most appropriate medium of multiplication culture was MS+5 mg·L^(-1)6-BA+0.4 mg·L^(-1) NAA,and a maximum multiplication coefficient was 6.20,and stable multiplication effect within three generations.The most appropriate medium of rooting culture was 1/2 MS+0.9 mg·L^(-1) IBA,and a maximum rooting rate was 94.44%,rooting number was 11.40,roots length was 2.94.The survival rate of transplanting after refining seedlings reaches 88.33%.This study provides technical support for factory seedling of Actinidia chinensis var.deliciosa’cuixiang’.
作者
丁光雪
杨迪
辛纳慧
李钰媛
张乃群
DING Guang-xue;YANG Di;XIN Na-hui;LI Yu-yuan;ZHANG Nai-qun(Nanyang Infant Normal School,Nanyang 474150,China;Nanyang Vocational College of Agriculture,Nanyang 473000,China;College of Life Science and Agriculture Engineering/Nanyang Normal University,Nanyang 473061,China)
出处
《山东农业大学学报(自然科学版)》
北大核心
2021年第3期388-393,共6页
Journal of Shandong Agricultural University:Natural Science Edition
基金
河南省科技攻关项目(102102110159)
河南省科技攻关计划项目(172102110108)。
关键词
‘翠香’猕猴桃
离体培养
育苗
Actinidia chinensis var.deliciosa’cuixiang’
in vitro
seeding