‘翠香’猕猴桃离体再生研究

Study on the Regeneration in Vitro of Actinidia chinensis var.deliciosa’cuixiang’

本文以‘翠香’猕猴桃带腋芽茎段为外植体,研究了不同激素配比对‘翠香’猕猴桃茎段腋芽诱导、增殖培养、生根培养的影响,并进行移栽。研究表明:‘翠香’猕猴桃最优的茎段腋芽诱导培养基为MS+2.0 mg·L^(-1)6-BA+0.3mg·L^(-1)NAA,诱导率为88.89%;最优的增殖培养培养基为MS+5 mg·L^(-1)6-BA+0.4 mg·L^(-1) NAA,增殖系数达6.20,三代连续增殖稳定;生根培养的最佳培养基为1/2MS+0.9 mg·L^(-1) IBA,生根率为94.44%,根数为11.40,根长为2.94;炼苗后移栽成活率达88.33%。此研究为‘翠香’猕猴桃的组织培养工厂化育苗提供了技术支持。

The stems with axillary buds of Actinidia chinensis var.deliciosa’cuixiang’was used as explants to study the effects of combinations of different plant growth regulator on induction of axillary buds,multiplication culture,rooting culture,and of transplanting.The most appropriate medium of induction of axillary buds was MS+2.0 mg·L^(-1)6-BA+0.3 mg·L^(-1) NAA,and a maximum induction rate was 88.89%.The most appropriate medium of multiplication culture was MS+5 mg·L^(-1)6-BA+0.4 mg·L^(-1) NAA,and a maximum multiplication coefficient was 6.20,and stable multiplication effect within three generations.The most appropriate medium of rooting culture was 1/2 MS+0.9 mg·L^(-1) IBA,and a maximum rooting rate was 94.44%,rooting number was 11.40,roots length was 2.94.The survival rate of transplanting after refining seedlings reaches 88.33%.This study provides technical support for factory seedling of Actinidia chinensis var.deliciosa’cuixiang’.