摘要
Mandarin fish(Siniperca chuatsi)is an economically important freshwater fish cultured in China.In this species,females grow faster than males.However,due to the lack of available genomic and transcriptome information,the mechanisms of sex differentiation remain poorly understood.In this study,Illumina high-throughput sequencing technology was used to sequence four cDNA libraries;the tissues examined included hypothalamus,pituitary gland,ovary,and testis.A total of 134124 high-quality unigenes were obtained on average length of 1361 bp and N50 of 3312 bp.A search of all-unigene sequences against NR,NT,SwissProt,KOG,KEGG,GO,and InterPro databases resulted in 59688(44.50%),76329(56.91%),50432(37.60%),45741(34.10%),48760(36.35%),5241(3.91%),and 46099(34.37%)annotations,respectively.In a comparison of ovarian and testicular libraries,15289 ovary-biased genes and 10035 testis-biased genes were identified,including a series of genes related to sex determination and differentiation,such as cyp19a1a,foxl2,sox9,dmrt1,amh,and others.In addition,49495 SSRs and 85899 SNPs were detected in transcriptome data.Quantitative real-time PCR results of 15 sex-related functional genes indicated that RNA-seq data was reliable.This study will contribute to a better understanding of the molecular mechanisms of sex differentiation and development in Mandarin fish.
基金
Supported by the National Key R&D Program of China(No.2018YFD0901203)
the Guangdong Basic and Applied Basic Research Foundation(No.2019B1515120072)
the Science and Technology Planning Project of Guangzhou(No.201904020043)。
