试管外生根对蓝莓生长发育的影响

Effect of Ex Vitro Rooting for the Test-tube Seedlings on the Growth and Development of Blueberry

以蓝莓组培苗为试验材料,研究比较了组培苗瓶内生根与瓶外扦插生根对蓝莓生长发育的影响。试验结果表明:两种生根方法获得的蓝莓幼苗,其净光合速率、蒸腾速率、胞间CO_(2)浓度、气孔导度等光合特征参数均无显著性差异;在水分利用效率、叶绿素含量、可溶性糖含量与可溶性蛋白含量等指标上也无显著性差异;幼苗主茎粗、新生枝数、主茎长、叶色指数等各生长指标亦无显著性差异。取带有5~6片展开叶的试管芽顶芽进行瓶外扦插生根,移栽成活率达到88%,说明试管芽瓶外扦插生根是培育蓝莓幼苗的一种可行方法,可用作蓝莓繁殖。

It was studied that the effects of the in vitro and ex vitro rooting for the test-tube seedlings on the growth and development of blueberry. The results showed that there were no significant differences at net photosynthetic rate, transpiration rate, intercellular CO_(2) concentration and stomatal conductance. There were also no significant difference at water use efficiency, chlorophyll content, soluble sugar content and soluble protein content. There were no significant differences at growth indicators including main stem diameter, number of new shoots, main stem length and leaf color index. The survival rate of transplanting reached 88% when the shoot buds with 5 to 6 pieces of expanded leaves were taken for rooting outside the bottle. The ex vitro rooting is a feasible method for the test-tube seedlings of blueberry.