摘要
依据SN/T 4091-2015《食品微生物学测量不确定度评估指南》和JJF 1059.1-2012《测量不确定度评定与表示》,分析影响样品中乳酸菌平板计数检验结果的不确定度来源,计算合成不确定度和扩展不确定度。结果表明,不确定度来源的主要因素所占分量由大到小排序为:重复性检测>样品稀释>加样>样品制备>环境温度。检验结果的扩展不确定度为6.2×10^(7)CFU/g,取值区间为6.0×10^(8)~7.3×10^(8)CFU/g,包含因子k=2。此方法适用于类似条件下乳酸菌计数不确定度的评定。
According to SN/T 4091-2015"Guidelines for the Evaluation of Uncertainty in Food Microbiology Mea-surement"and JJF 1059.1-2012"Evaluation and Expression of Determination Uncertainty",the sources of uncer-tainty affecting the determination results of lactobacillus tablets in the traditional fermented primrose were analyzed,and the uncertainty of synthesis and expansion were calculated.The results show that during the inspection process,the components of the various factors of the uncertain source are ranked from large to small:repeatability test>sam-ple dilution>sample addition>sample preparation>environment.The extended uncertainty of the lactic acid bac-teria test results is 6.2×10^(7) CFU/g,and the lactic acid bacteria plate count test results range from 6.0×10^(8) to 7.3×10^(8)C FU/g,including the factor k=2.This method is suitable for evaluating the uncertainty of lactic acid bacteria countunder similar conditions.
作者
刘笑笑
李胜男
樊慧梅
魏春雁
LIU Xiaoxiao;LI Shengnan;FAN Huimei;WEI Chunyan(Institute of Agricultural Quality Standards and Testing Technology,Jilin Academy of Agricultural Sciences/Risk Assessment Lab of Agri-products Quality and Safety(Changchun),Agriculture Ministry of P.R.C.,Changchun 130033,China)
出处
《东北农业科学》
2021年第3期126-129,共4页
Journal of Northeast Agricultural Sciences
基金
吉林省农业科技创新工程项目(CXGC2018ZY004、CXGC2017ZY038、CXGC2017TD011)。
关键词
发酵樱菜
乳酸菌
平板计数法
不确定度
Fermentated Lepidium sativum
Lactobacillus
Tablet counting method
Uncertainty evaluation