基于海马区BDNF的变化研究电针调控外源性NSCs治疗PSCI的作用及机制

Study on the effect and mechanism of electroacupuncture regulating exogenous NSCs to treat PSCI based on changes in BDNF in the hippocampus

目的观察电针调控外源性神经干细胞(NSCs)治疗卒中后认知障碍(PSCI)的作用,并基于海马区脑源性神经营养因子(BDNF)的变化探讨其作用机制。方法将24只大脑中动脉闭塞模型(MCAO)大鼠配对分为模型组(8只),NSCs移植组(8只),电针+NSCs移植组(8只);由于造模后部分大鼠死亡,最终各组取6只大鼠进行数据分析。模型组大鼠给予基础饲养以及同等条件抓取固定,NSCs移植组采用NSCs移植治疗,电针+NSCs移植组在NSCs移植基础上行电针百会穴治疗。采用Morris水迷宫实验观察大鼠学习记忆能力;采用蛋白免疫印迹法(Westernblot)检测大鼠海马区BDNF蛋白的表达情况。结果与模型组相比,NSCs移植组大鼠逃避潜伏期时间缩短,差异均有统计学意义(均P<0.05);与NSCs移植组及模型组相比,电针+NSCs移植组大鼠逃避潜伏期时间缩短,差异均有统计学意义(均P<0.05)。电针+NSCs移植组大鼠跨越平台次数为(2.28±0.35)次,高于NSCs移植组(1.76±0.46)次与模型组(0.98±0.32)次,差异均有统计学意义(均P<0.05)。电针+NSCs移植组大鼠海马区BDNF蛋白表达量为(1.45±0.35),明显高于NSCs移植组(0.92±0.22)、模型组(0.86±0.25),差异均有统计学意义(均P<0.05);NSCs移植组大鼠海马区BDNF蛋白表达量与模型组比较,差异无统计学意义(P>0.05)。结论电针可能基于调节海马区BDNF的表达调控外源性NSCs从而改善PSCI。

Objective To observe the effect of electroacupuncture regulating exogenous neural stem cells(NSCs)in the treatment of post-stroke cognitive impairment(PSCI),and to explore its mechanism based on change in brain-derived neurotrophic factor(BDNF)in the hippocampus.Methods Twenty-four rats with middle cerebral artery occlusion(MCAO)were divided into a model group,a NSCs transplantation group,and an electroacupuncture+NSCs transplantation group by the paired design,with 8 rats in each group.Since some rats died after modeling,6 rats were selected from each group for data analysis.The rats in the model group were given basic feeding and grasping under the same conditions.The NSCs transplantation group was treated with NSCs transplantation,and the electroacupuncture+NSCs transplantation group was treated with electroacupuncture at Baihui acupoint after NSCs transplantation.Morris water maze experiment was used to observe the learning and memory abilities of rats;Western blot technology was used to detect the expression of BDNF protein in the hippocampus of rats.Results Compared with the model group,the rats in the NSCs transplantation group had shorter escape latency(all P<0.05);compared with the model group and the NSCs transplantation group,the rats in the electroacupuncture+NSCs transplantation group had shorter escape latency(all P<0.05).The number of crossing platforms of rats in the electroacupuncture+NSCs transplantation group was(2.28±0.35)times,which was higher than those in the model group and the NSCs transplantation group[(0.98±0.32)and(1.76±0.46)times](both P<0.05).The expression of BDNF protein in the hippocampus of rats in the electroacupuncture+NSCs transplantation group was(1.45±0.35),which was higher than those in the model group and the NSCs transplantation group[(0.86±0.25)and(0.92±0.22)](both P<0.05);there was no statistically significant difference in the expression of BDNF protein in the hippocampus of rats in the model group and the NSCs transplantation group(P>0.05).Conclusion Electroacupuncture may regulate exogenous NSCs to improve PSCI based on regulating the expression of BDNF in the hippocampus.