乳铁蛋白对炎症性肠炎大鼠肠道微生态的正向调节作用

Positive Regulation of Lactoferrin on Gut Micro-Ecology of Rats with Inflammatory Bowel Disease

本研究采用葡聚糖硫酸钠(dextran sulphate sodium,DSS)刺激以构建SD大鼠炎症性肠炎(inflammatory bowel disease,IBD)模型,通过高通量测序分析乳铁蛋白(lactoferrin,LF)对大鼠结肠粪便肠道菌群α-多样性、物种差异性分布、KEGG信号通路相关操作分类单元(operational taxonomic unit,OTU)丰度的影响。结果表明,LF有助于缓解DSS刺激所引起的α-多样性降低和优势菌群——厚壁菌门(Firmicutes)与拟杆菌门(Bacteroidetes)的比例失调;与DSS组相比,高剂量LF提高了患IBD大鼠乳杆菌科(Lactobacillaceae)(12%vs.40%)、瘤胃菌科(Ruminococcaceae)(17%vs.27%)等菌群的相对丰度,降低了拟杆菌科(Bacteroides)(28%vs.8%)、普雷沃氏菌科(Prevotellaceae)(13%vs.6%)等菌群的相对丰度。KEGG信号通路OTU丰度分析表明,LF的摄入降低了患IBD大鼠菌群中与癌症、感染性疾病等人类疾病、免疫系统和细胞及遗传相关信号通路的OTU丰度,并降低了这些疾病的发生风险。综上,LF的摄入对患IBD大鼠结肠微生态具有正向调节作用,可使其肠道菌群的组成更接近于健康大鼠,且有降低其他伴随性疾病风险的可能。

The effect of lactoferrin(LF)on theα-diversity and composition,as well as the abundance of KEGG signaling pathway-related operational taxonomic unit(OTU)in the colonic microbiota of rats with inflammatory bowel disease(IBD)induced by dextran sulphate sodium(DSS)was analyzed by high-throughput sequencing.The results showed that LF alleviated the reduction ofα-diversity and the imbalance between the dominant phyla Firmicutes and Bacteroidetes caused by DSS stimulation.LF increased the abundance of Lactobacillaceae(12%vs.40%)and Ruminococcaceae(17%vs.27%),and decreased Bacteroides(28%vs.8%)and Prevotellaceae(13%vs.6%).LF administration reduced the abundance of OTUs associated with human diseases such as cancer and infectious diseases,immune system,cells and genetics-related signaling pathways in the gut microbiota in IBD rats.The results of this study indicated that LF can play a beneficial role in regulating the colonic micro-ecology of IBD rats and modulating the microbiota composition to be similar to that of healthy rats,thereby having the potential to reduce the risk of other concomitant diseases.